 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 17, 2008 |
| Title |
The response of Saccharomyces cerevisiae to stress throughout a 15-day wine fermentation |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
We used genome-wide expression analyses to study the response of Saccharomyces cerevisiae to stress throughout a 15-day wine fermentation. Forty percent of the yeast genome significantly changed expression levels to mediate long-term adaptation to an environment in which ethanol is both a stressor and a carbon source. Within this set, we identify a group of 223 genes, designated as the Fermentation Stress Response (FSR), that are dramatically and permanently induced; FSR genes exhibited changes ranging from four-to eighty-fold. The FSR is novel; 62% of the genes involved have not been implicated in global stress responses and 28% of the genes have no functional annotation. Genes involved in respiratory metabolism and gluconeogenesis were expressed during fermentation despite the presence of high concentrations of glucose. Ethanol, rather than nutrient depletion, was responsible for entry of yeast cells into stationary phase. Ethanol seems to regulate yeast metabolism through hitherto undiscovered regulatory networks during wine fermentation.
Keywords: time course, stress response, fermentation
|
| |
|
| Overall design |
The main study is based on expression measurements at 0.5,2,3.5,7 and 10 % ethanol (corresponding to roughly 24,48,60,120 and 340 hours). Experiments were done in triplicate (biological replicates). This submission also includes the files at 1 hour and at 12 hours for completeness. (also in triplicate)
|
| |
|
| Contributor(s) |
D Marks V, Ho Sui SJ, Erasmus D, van der Merwe GK, Brumm J, Wasserman WW, Bryan J, van Vuuren HJ |
| Citation(s) |
18215224 |
| Submission date |
Jul 20, 2007 |
| Last update date |
Jul 01, 2016 |
| Contact name |
Hennie JJ van Vuuren |
| E-mail(s) |
hjjvv@interchange.ubc.ca
|
| Phone |
(604) 822-0418
|
| Fax |
(604) 822-5143
|
| URL |
http://www.landfood.ubc.ca/wine/
|
| Organization name |
UBC
|
| Street address |
2205 East Mall
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL90 |
[YG_S98] Affymetrix Yeast Genome S98 Array |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA101673 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8536_RAW.tar |
38.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...