|
| Status |
Public on Dec 27, 2007 |
| Title |
BY4742 Quiescent and Non-quiescent replicates |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Cells in glucose-limited Saccharomyces cerevisiae cultures differentiate into quiescent (Q) and non-quiescent (NQ) fractions prior to entering stationary phase. To identify genes involved in this differentiation, Q and NQ cells from 101 deletion-mutant strains were tested for viability and reproductive capacity. Twenty-one mutants were identified, including 7 that affected reproductive capacity of both cell types. Thirteen affected only Q or NQ cells, indicating significant differentiation of these cell types. doa4 strains, lacking ubiquitin hydrolase, affected viability and reproductive capacity in both cell types. More than 1300 mRNAs differentiating Q and NQ cell fractions were identified by microarray analysis. Gene-ontology analysis of Q-cell mRNAs showed significant increases in protein-encoding mRNAs involved in membrane maintenance, oxidative stress response, and signal transduction. NQ-cell mRNAs encode proteins involved in Ty-element transposition and DNA recombination, consistent with apoptosis in these cells. Consistent with preparation for rapid response to environmental stimuli, approximately 2000 protease-labile mRNAs were identified in Q cells. The differentiation of these cell types and the ability of genes to selectively affect the survival of Q or NQ cells in yeast are relevant to chronological aging, cell-cycle, genome-evolution, and stem-cell research and provides insight into complex responses that even simple organisms have to starvation.
Keywords: Cell-type comparison
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| |
|
| Overall design |
10 replicates per cell-type. Channel 1 (Cy3) was the control of mixture of exponential and stationary phase cells. Channel 2 (Cy5) was the experimental cell-types of parental strain BY4742.
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| |
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| Contributor(s) |
Aragon AD, Werner-Washburne M |
| Citation(s) |
18199684 |
| |
| Submission date |
Jul 20, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Sushmita Roy |
| E-mail(s) |
sroy@cs.unm.edu
|
| Phone |
5052779340
|
| Organization name |
Biology, University of New Mexico
|
| Street address |
|
| City |
Albuquerque |
| State/province |
NM |
| ZIP/Postal code |
87106 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5627 |
Yeast 70mer oligoset by4742 |
|
| Samples (20)
|
| GSM211730 |
BY4742 Quiescent time (Replicate 1) |
| GSM211731 |
BY4742 Quiescent time (Replicate 2) |
| GSM211732 |
BY4742 Quiescent time (Replicate 3) |
| GSM211733 |
BY4742 Quiescent time (Replicate 4) |
| GSM211734 |
BY4742 Quiescent time (Replicate 5) |
| GSM211735 |
BY4742 Quiescent time (Replicate 6) |
| GSM211736 |
BY4742 Quiescent time (Replicate 7) |
| GSM211737 |
BY4742 Quiescent time (Replicate 8) |
| GSM211738 |
BY4742 Quiescent time (Replicate 9) |
| GSM211739 |
BY4742 Quiescent time (Replicate 10) |
| GSM211746 |
BY4742 Non-quiescent time (Replicate 1) |
| GSM211747 |
BY4742 Non-quiescent time (Replicate 2) |
| GSM211748 |
BY4742 Non-quiescent time (Replicate 3) |
| GSM211749 |
BY4742 Non-quiescent time (Replicate 4) |
| GSM211750 |
BY4742 Non-quiescent time (Replicate 5) |
| GSM211751 |
BY4742 Non-quiescent time (Replicate 6) |
| GSM211752 |
BY4742 Non-quiescent time (Replicate 7) |
| GSM211753 |
BY4742 Non-quiescent time (Replicate 8) |
| GSM211754 |
BY4742 Non-quiescent time (Replicate 9) |
| GSM211755 |
BY4742 Non-quiescent time (Replicate 10) |
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| This SubSeries is part of SuperSeries: |
| GSE8624 |
Quiescent and non-quiescent deletion-mutant strains |
|
| Relations |
| BioProject |
PRJNA105371 |
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