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Status |
Public on Dec 27, 2007 |
Title |
S288c Quiescent and Non-quiescent replicates |
Organism |
Saccharomyces cerevisiae |
Experiment type |
Expression profiling by array
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Summary |
Cells in glucose-limited Saccharomyces cerevisiae cultures differentiate into quiescent (Q) and non-quiescent (NQ) fractions prior to entering stationary phase. To identify genes involved in this differentiation, Q and NQ cells from 101 deletion-mutant strains were tested for viability and reproductive capacity. Twenty-one mutants were identified, including 7 that affected reproductive capacity of both cell types. Thirteen affected only Q or NQ cells, indicating significant differentiation of these cell types. doa4 strains, lacking ubiquitin hydrolase, affected viability and reproductive capacity in both cell types. More than 1300 mRNAs differentiating Q and NQ cell fractions were identified by microarray analysis. Gene-ontology analysis of Q-cell mRNAs showed significant increases in protein-encoding mRNAs involved in membrane maintenance, oxidative stress response, and signal transduction. NQ-cell mRNAs encode proteins involved in Ty-element transposition and DNA recombination, consistent with apoptosis in these cells. Consistent with preparation for rapid response to environmental stimuli, approximately 2000 protease-labile mRNAs were identified in Q cells. The differentiation of these cell types and the ability of genes to selectively affect the survival of Q or NQ cells in yeast are relevant to chronological aging, cell-cycle, genome-evolution, and stem-cell research and provides insight into complex responses that even simple organisms have to starvation. Keywords: cell type comparison
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Overall design |
10 replicates per cell-type. Channel 1 (Cy3) was the control of mixture of exponential and stationary phase cells. Channel 2 (Cy5) was the experimental cell-types of wild-type strain S288c.
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Contributor(s) |
Aragon AD, Werner-Washburne M |
Citation(s) |
18199684 |
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Submission date |
Jul 23, 2007 |
Last update date |
Mar 19, 2012 |
Contact name |
Sushmita Roy |
E-mail(s) |
sroy@cs.unm.edu
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Phone |
5052779340
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Organization name |
Biology, University of New Mexico
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Street address |
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City |
Albuquerque |
State/province |
NM |
ZIP/Postal code |
87106 |
Country |
USA |
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Platforms (1) |
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Samples (20)
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GSM211759 |
S288C Quiescent time (Replicate 4) |
GSM211760 |
S288C Quiescent time (Replicate 5) |
GSM211761 |
S288C Quiescent time (Replicate 6) |
GSM211762 |
S288C Quiescent time (Replicate 7) |
GSM211763 |
S288C Quiescent time (Replicate 8) |
GSM211764 |
S288C Quiescent time (Replicate 9) |
GSM211765 |
S288C Quiescent time (Replicate 10) |
GSM211766 |
S288C Non-quiescent time (Replicate 1) |
GSM211767 |
S288C Non-quiescent time (Replicate 2) |
GSM211768 |
S288C Non-quiescent time (Replicate 3) |
GSM211769 |
S288C Non-quiescent time (Replicate 4) |
GSM211770 |
S288C Non-quiescent time (Replicate 5) |
GSM211771 |
S288C Non-quiescent time (Replicate 6) |
GSM211772 |
S288C Non-quiescent time (Replicate 7) |
GSM211773 |
S288C Non-quiescent time (Replicate 8) |
GSM211774 |
S288C Non-quiescent time (Replicate 9) |
GSM211775 |
S288C Non-quiescent time (Replicate 10) |
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This SubSeries is part of SuperSeries: |
GSE8624 |
Quiescent and non-quiescent deletion-mutant strains |
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Relations |
BioProject |
PRJNA105377 |