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| Status |
Public on Dec 20, 2017 |
| Title |
Whole transcriptome profiling reveals major cell types in the cellular immune response against acute and chronic active Epstein-Barr virus infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Epstein–Barr virus (EBV) is a common human pathogen that infects over 95% of the population worldwide. In the present study, the whole transcriptome microarray data were generated from peripheral blood mononuclear cells from Chinese children with acute infectious mononucleosis (AIM) and chronic active EBV infection (CAEBV) that were also compared with a publicly available microarray dataset from a study of American college students with AIM. Our study characterized for the first time a broad spectrum of molecular signatures in AIM and CAEBV. The key findings from the transcriptome profiling were validated with qPCR and flow cytometry assays. The most important finding in our study is the discovery of predominant γδ TCR expression and γδ T cell expansion in AIM. This finding, in combination with the striking up-regulation of CD3, CD8 and CD94, suggests that CD8+ T cells and CD94+ NK cells may play a major role in AIM. Moreover, the unique up-regulation of CD64A/B and its significant correlation with the monocyte marker CD14 was observed in CAEBV and that implies an important role of monocytes in CAEBV. In conclusion, our study reveals major cell types (particularly γδ T cells) in the host cellular immune response against AIM and CAEBV.
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| Overall design |
Total RNAs were extracted from peripheral blood mononulear cells from 6 acute infectious mononucleosis (AIM), 5 chronic active EBV infection (CAEBV) and 6 healthy controls. Differential expression was assessed using the R package "limma", and pathway enrichment was performed using the R package "GAGE". Expression signal correlation with 36 blood cell markers was defined by Pearson correlation analysis.
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| Contributor(s) |
Yu J, Zhong H, Xu J, Hu X |
| Citation(s) |
29259291 |
| Submission date |
Aug 15, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Jinsheng Yu |
| E-mail(s) |
jyu@wustl.edu
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| Organization name |
Washington University School of Medicine
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| Department |
Genetics
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| Lab |
GTAC Lab
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| Street address |
660 S. Euclid Ave.
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| City |
St. Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (17)
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| Relations |
| BioProject |
PRJNA338922 |
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