|
| Status |
Public on May 30, 2018 |
| Title |
Genome-wide mapping of the Arabidopsis thaliana heat shock transcription factor A1b binding sites under non-stress and heat stress conditions [ChIP-seq] |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We used illumina-based next generation sequencing technology to to identify the regions bound by HSFA1b in the Arabidopsis genome. We sequenced HSFA1b chromatin immunoprecipitated genomic sequences under non-stress and heat stress conditions to understand the changes in the HSFA1b binding map when the growth conditions are switched from favorable to heat stress. We show that the binding map of HSFA1b in the Arabidopsis genome is subject to reconfiguration when the growth conditions are switched from non-stress to heat stress response. We also show that HSFA1b is targeting genes involved in developmental processes beside genes involved in stress response under both conditions indicating that HSFA1b possibly regulates the expression of both developmental and stress genes under non-stress and under heat stress, possibly for a limited duration prior heat acclimation.
|
| |
|
| Overall design |
Mapping the binding sites if HSFA1b in the Arabidopsis genome under non-stress and heat stress conditions
|
| |
|
| Contributor(s) |
Albihlal WS, Mullineaux PM |
| Citation(s) |
29697803 |
| |
| Submission date |
Aug 15, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Philip M Mullineaux |
| E-mail(s) |
mullin@essex.ac.uk
|
| Phone |
+44 (0) 1206 872118
|
| Organization name |
University of Essex
|
| Department |
Environmental and Plant Bioscience Research Group
|
| Lab |
5.36
|
| Street address |
Wivenhoe Park
|
| City |
Colchester |
| State/province |
Essex |
| ZIP/Postal code |
CO4 3SQ |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE85655 |
Genome-wide mapping of the Arabidopsis thaliana heat shock transcription factor A1b binding sites under non-stress and heat stress conditions |
|
| Relations |
| BioProject |
PRJNA339000 |
| SRA |
SRP082176 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE85651_35S_heat_stress_PE_peaks.narrowPeak.gz |
213.2 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE85651_35S_heat_stress_PE_peaks.xls.gz |
260.8 Kb |
(ftp)(http) |
XLS |
| GSE85651_35S_heat_stress_PE_summits.bed.gz |
142.9 Kb |
(ftp)(http) |
BED |
| GSE85651_35S_no_stress_PE_peaks.narrowPeak.gz |
333.2 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE85651_35S_no_stress_PE_peaks.xls.gz |
404.1 Kb |
(ftp)(http) |
XLS |
| GSE85651_35S_no_stress_PE_summits.bed.gz |
222.9 Kb |
(ftp)(http) |
BED |
| GSE85651_native_heat_stress_peaks.narrowPeak.gz |
26.7 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE85651_native_heat_stress_peaks.xls.gz |
31.7 Kb |
(ftp)(http) |
XLS |
| GSE85651_native_heat_stress_summits.bed.gz |
16.3 Kb |
(ftp)(http) |
BED |
| GSE85651_native_no_stress_peaks.narrowPeak.gz |
23.9 Kb |
(ftp)(http) |
NARROWPEAK |
| GSE85651_native_no_stress_peaks.xls.gz |
28.2 Kb |
(ftp)(http) |
XLS |
| GSE85651_native_no_stress_summits.bed.gz |
13.4 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
Less...
More...