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| Status |
Public on Aug 02, 2017 |
| Title |
Genetic profiling of developing human embryonic stem cell derived Retinal pigmented epithelium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Age-related macular degeneration is a progressive disease resulting in impaired central vision. Degeneration of the retinal pigmented epithelial (RPE) monolayer is associated with the progression of the disease. To date no treatment is able to stop this progression, however new cell replacement studies using human embryonic stem cells (hESC) to generate RPE show a promising prospective. To improve cell replacement strategies a better understanding about the development of RPE cells is necessary. In the current study we therefore do extensive genetic profiling of hESC derived RPE cells at different stages during development based on their pigmentation.
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| Overall design |
Start pigmentation samples (n=6, Cy5) were compared with late pigmentation samples (n=6, Cy5). Data was normalized on pooled common reference (Cy3) expression using R LIMMA
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| Contributor(s) |
Bennis A, Bergen AA |
| Citation(s) |
28730556 |
| |
| Submission date |
Aug 22, 2016 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Anna Bennis |
| E-mail(s) |
a.bennis@amc.uva.nl
|
| Organization name |
Amsterdam Medical Centre Amsterdam
|
| Department |
Clinical Genetics
|
| Street address |
Meibergdreef 9
|
| City |
Amsterdam |
| ZIP/Postal code |
1105 AZ |
| Country |
Netherlands |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA339736 |
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