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Series GSE85918 Query DataSets for GSE85918
Status Public on Aug 23, 2016
Title APOL1 renal-risk variants induce mitochondrial dysfunction (Illumina)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To elucidate pathways whereby apolipoprotein L1 gene (APOL1) G1 and G2 variants facilitate kidney disease in African Americans, human embryonic kidney cells (HEK293) were used to establish doxycycline-inducible (Tet-on) cell lines stably expressing reference APOL1 G0 and its G1 and G2 renal-risk variants. Illumina human HT-12-v4 arrays and Affymetrix HTA 2.0 arrays were employed to generate global gene expression data with doxycycline induction. Significantly altered pathways identified through bioinformatics involved mitochondrial function; results were validated using immunoblotting, immunofluorescence and functional assays.
 
Overall design Global gene expression profiles were performed on HEK293 Tet-on G0, G1, G2 and empty vector cells with and without Dox induction using Illumina human HT-12 v4 arrays. Another independent gene expression array system, Affymetrix HTA 2.0, was used to verify the results of Illumina arrays. Pair-wise and pattern-based analyses were applied to detect the mostly impacted pathways due to overexpression and by APOL1 genotypes.
 
Contributor(s) Ma L, Chou JW, Snipes JA, Bharadwaj MS, Craddock AL, Cheng D, Weckerle A, Petrovic S, Hicks PJ, Hemal AK, Hawkins GA, Miller LD, Molina AA, Langefeld CD, Murea M, Parks JS, Freedman BI
Citation(s) 27821631
Submission date Aug 22, 2016
Last update date Aug 13, 2018
Contact name Lijun Ma
E-mail(s) lima@wakehealth.edu
Organization name Wake Forest School of Medicine
Department Nephrology
Street address 1 Medical Center Blvd
City Winston Salem
State/province NC
ZIP/Postal code 27157
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (24)
GSM2287798 HEK293_Tet-on G0(-)_1
GSM2287799 HEK293_Tet-on G0(-)_2
GSM2287800 HEK293_Tet-on G0(-)_3
This SubSeries is part of SuperSeries:
GSE85921 APOL1 renal-risk variants induce mitochondrial dysfunction
Relations
BioProject PRJNA339760

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE85918_RAW.tar 64.6 Mb (http)(custom) TAR (of IDAT)
GSE85918_non-normalized.txt.gz 6.9 Mb (ftp)(http) TXT
Processed data included within Sample table

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