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| Status |
Public on Aug 23, 2016 |
| Title |
APOL1 renal-risk variants induce mitochondrial dysfunction (Affymetrix 1) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To elucidate pathways whereby apolipoprotein L1 gene (APOL1) G1 and G2 variants facilitate kidney disease in African Americans, human embryonic kidney cells (HEK293) were used to establish doxycycline-inducible (Tet-on) cell lines stably expressing reference APOL1 G0 and its G1 and G2 renal-risk variants. Illumina human HT-12-v4 arrays and Affymetrix HTA 2.0 arrays were employed to generate global gene expression data with doxycycline induction. Significantly altered pathways identified through bioinformatics involved mitochondrial function; results were validated using immunoblotting, immunofluorescence and functional assays.
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| Overall design |
Global gene expression profiles were performed on HEK293 Tet-on G0, G1, G2 and empty vector cells with and without Dox induction using Illumina human HT-12 v4 arrays. Another independent gene expression array system, Affymetrix HTA 2.0, was used to verify the results of Illumina arrays. Pair-wise and pattern-based analyses were applied to detect the mostly impacted pathways due to overexpression and by APOL1 genotypes.
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| Contributor(s) |
Ma L, Chou JW, Snipes JA, Bharadwaj MS, Craddock AL, Cheng D, Weckerle A, Petrovic S, Hicks PJ, Hemal AK, Hawkins GA, Miller LD, Molina AA, Langefeld CD, Murea M, Parks JS, Freedman BI |
| Citation(s) |
27821631 |
| |
| Submission date |
Aug 22, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Lijun Ma |
| E-mail(s) |
lima@wakehealth.edu
|
| Organization name |
Wake Forest School of Medicine
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| Department |
Nephrology
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| Street address |
1 Medical Center Blvd
|
| City |
Winston Salem |
| State/province |
NC |
| ZIP/Postal code |
27157 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE85921 |
APOL1 renal-risk variants induce mitochondrial dysfunction |
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| Relations |
| BioProject |
PRJNA339759 |
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