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| Status |
Public on Aug 27, 2016 |
| Title |
Developmental Vitamin D availability impacts hematopoietic stem cell production |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Vitamin D insufficiency is a global epidemic and despite its high incidence the impact of Vitamin D signaling beyond calcium-bone regulation is not fully understood. To expand our analysis of the impact of 1,25(OH)D3 on hematopoietic stem and progenitor cell (HSPC) production and function in zebrafish and human umbilical cord blood (hUCB), we performed a microarray study to indentify signaling pathways downstream of 1,25(OH)D3 stimulation in hUCB CD34+ cells.
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| Overall design |
Microarray analysis was performed to identify downstream targets of Vitamin D signaling in human umbilical cord blood (hUCB) CD34+ cells. We compared the expression profile of hUCB HSCs, split and treated ex vivo for 4 hrs with 1,25(OH)D3 at 1mM, with matched vehicle (DMSO) controls. This treatment lead to the identification of 407 differentially expressed genes.
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| Contributor(s) |
Cortes M, North TE, Ho SS, Mistry M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Aug 26, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Trista E North |
| Organization name |
Beth Israel Deaconess Medical Center, Harvard Medical School
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| Department |
Pathology
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| Street address |
3 Blackfan Circle
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| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (4)
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| GSM2293538 |
hUCBCD34+_DMSO_444_Biological replicate 1 |
| GSM2293539 |
hUCBCD34+_DMSO_394_Biological replicate 2 |
| GSM2293540 |
hUCBCD34+_1,25(OH)D3_444_Biological replicate 1 |
| GSM2293541 |
hUCBCD34+_1,25(OH)D3_394_Biological replicate 2 |
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| Relations |
| BioProject |
PRJNA340259 |
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