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| Status |
Public on May 15, 2017 |
| Title |
Transcription and chromatin determinants of the rate of de novo DNA methylation in oocytes |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
We generated genome-wide methylation and transcriptome maps of size-selected, growing oocytes to capture the onset and progression of methylation. We find that the major transitions in the oocyte transcriptome occur well before the de novo methylation phase; nevertheless, transcription level does correlate with rate of methylation. Conversely, timing of methylation of CpG islands (CGIs) correlates inversely with enrichment of histone modifications inhibitory to DNA methylation and dependence on histone 3 lysine-4 demethylases, implicating chromatin remodelling as a major determinant of methylation timing.
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| |
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| Overall design |
RNA-Seq, PBAT and RRBS in mouse oocytes at different stages of folliculogenesis
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| |
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| Contributor(s) |
Veselovska L, Tomizawa S, Smallwood S, Saadeh H, Kelsey G |
| Citation(s) |
28507606 |
|
Lenka Gahurova, Shin-ichi Tomizawa, Sébastien A. Smallwood, Kathleen R. Stewart-Morgan, Heba Saadeh, Jeesun Kim, Simon R. Andrews, Taiping Chen and Gavin Kelsey. Transcription and chromatin determinants of de novo DNA methylation timing in oocytes. Epigenetics & Chromatin 2017;10:25. doi: 10.1186/s13072-017-0133-5
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| |
| Submission date |
Aug 31, 2016 |
| Last update date |
Jul 11, 2019 |
| Contact name |
Gavin Kelsey |
| Organization name |
The Babraham Institute
|
| Street address |
Babraham Research Campus
|
| City |
Cambridge |
| ZIP/Postal code |
CB22 3AT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA341373 |
| SRA |
SRP083895 |
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