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| Status |
Public on Sep 07, 2019 |
| Title |
Genome-wide long non-coding RNAs and mRNAs analysis of the tissues related to ovarian endometriosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
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| Summary |
To investigate the function of long non-coding RNA (lncRNA) in ovarian endometriosis and explore its pathogenesis from a new prospective pathway, we conducted genome-wide microarray expression profiling.GO and KEGG analysis demonstrated that the differential mRNAs in ECvsEU and NOvsEU mostly focused on cell adhesion and biological adhesion pathways, nevertheless ECvsNO on molecular metabolism pathways. CHL1 was the most up-regulated mRNA in EC than EU, and the expression of its two antisense lncRNAs, CHL1-AS1 and CHL1-AS2 exhibited the same tendency. They were significantly correlated with each other through bioinformatic calculation.GSEA analysis revealed that CHL1-AS1 and CHL1-AS2 were conspicuously concerned with the same gene set. Validated by qRT-PCR, CHL1-AS2 was higher expressed in EC than EU. It was also up-regulated in NO, and the relative fold changes in EC/EU and NO/EU showed no statistical significance (P>0.05). Furthermore, the value of EC/NO was more closed to 1 when sample size was enlarged, presenting the near expression of CHL1-AS2 in EC and NO. Consequently, we supposed that CHL1, CHL1-AS1 and CHL1-AS2 may play an important part in ovarian endometriosis.
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| Overall design |
We collected ectopic endometrium (EC), normal ovarian (NO) and ectopic endometrium (EU) from four patients with ovarian endometriosis, and compared the lncRNA/mRNA expression files of these three kinds of tissues in pairs
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| Contributor(s) |
Zhang C, Wu W, Luo J, Guan J, Chang X |
| Citation(s) |
30496345 |
| |
| Submission date |
Sep 07, 2016 |
| Last update date |
Dec 07, 2019 |
| Contact name |
Chen Zhang |
| E-mail(s) |
zhangchen20080925@163.com
|
| Organization name |
Peking University People’s Hospital
|
| Department |
Gynecological Oncology Center
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| Street address |
NO.11, Xizhimen South Street
|
| City |
Beijing |
| ZIP/Postal code |
100044 |
| Country |
China |
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| Platforms (1) |
| GPL20115 |
Agilent-067406 Human CBC lncRNA + mRNA microarray V4.0 (Probe name version) |
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| Samples (12)
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| GSM2305282 |
Patient NO.27_ectopic endometrium_rep1 |
| GSM2305283 |
Patient NO.13_eutopic endometrium_rep1 |
| GSM2305284 |
Patient NO.21_eutopic endometrium_rep1 |
| GSM2305285 |
Patient NO.25_eutopic endometrium_rep1 |
| GSM2305286 |
Patient NO.27_eutopic endometrium_rep1 |
| GSM2305287 |
Patient NO.13_lesion adjacent tissue_rep1 |
| GSM2305288 |
Patient NO.21_lesion adjacent tissue_rep1 |
| GSM2305289 |
Patient NO.25_lesion adjacent tissue_rep1 |
| GSM2305290 |
Patient NO.27_lesion adjacent tissue_rep1 |
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| Relations |
| BioProject |
PRJNA342153 |
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