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| Status |
Public on Dec 03, 2007 |
| Title |
PrV/PK15 kinetics_SLA/PrV slides |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
To study in vitro the epithelial cells and PrV interactions during infection, we followed PrV and PK15 cells transcriptome modifications during time-course infection (I) and mock-infection (MI).Six time points were studied: just after I and MI, 1h, 2h, 4h, 8h and 12h post-I and MI. For this study, a pig DNA/cDNA microarray containing genes of the SLA region, additional genes encoding other important immunological molecules and all the PrV genes was constructed.
Keywords: infection time course
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| Overall design |
48 samples - The hybridization scheme which can be define as dye-switch was chosen. A balanced loop design with two independent loops, each loop containing 2 replicates of PrV infection and MI, was used. In total, 24 SLA/PrV slides (containing 2 networks) were used in this experiment.
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| Contributor(s) |
Flori L, Rogel-Gaillard C, Cochet M, Lemonnier G, Hugot K, Chardon P, Robin S, Lefèvre F |
| Citation missing |
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| |
| Submission date |
Aug 03, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
Laurence Flori |
| E-mail(s) |
laurence.flori@jouy.inra.fr
|
| Organization name |
INRA
|
| Department |
Génétique Animale
|
| Lab |
LREG
|
| Street address |
Domaine de Vilvert
|
| City |
Jouy en Josas |
| ZIP/Postal code |
78350 |
| Country |
France |
| |
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| Platforms (1) |
| GPL5622 |
SLA_PrV porcine DNA/cDNA microarray |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA101891 |
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