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Series GSE86824 Query DataSets for GSE86824
Status Public on Jan 31, 2019
Title RNA Sequencing of mouse Purkinje cells across postnatal development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We analyzed Purkinje cell transcriptome dynamics in the developing mouse cerebellum during the first three postnatal weeks, a key developmental period equivalent to the third trimester in human cerebellar development. Our study represents the first detailed analysis of developmental Purkinje cell transcriptomes and provides a valuable dataset for gene network analyses and biological questions on genes implicated in cerebellar and Purkinje cell development.
 
Overall design Laser capture microdissection was employed to obtain a highly enriched population of cerebellar Purkinje cells. Deep sequencing was performed on RNA isolated from 1000 Purkinje cells at five developmental timepoints (postnatal days P0, P4, P8, P14 and P21) in triplicate.
 
Contributor(s) Clifford H, Dulneva A, Ponting CP, Haerty W, Becker EB
Citation(s) 30679692
Submission date Sep 12, 2016
Last update date May 15, 2019
Contact name Esther Becker
E-mail(s) esther.becker@dpag.ox.ac.uk
Organization name University of Oxford
Street address Sherrington Road
City Oxford
ZIP/Postal code OX13PT
Country United Kingdom
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM2309043 P0_R1
GSM2309044 P0_R2
GSM2309045 P0_R3
Relations
BioProject PRJNA342640
SRA SRP089712

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE86824_RAW.tar 2.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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