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Status |
Public on Jan 31, 2019 |
Title |
RNA Sequencing of mouse Purkinje cells across postnatal development |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We analyzed Purkinje cell transcriptome dynamics in the developing mouse cerebellum during the first three postnatal weeks, a key developmental period equivalent to the third trimester in human cerebellar development. Our study represents the first detailed analysis of developmental Purkinje cell transcriptomes and provides a valuable dataset for gene network analyses and biological questions on genes implicated in cerebellar and Purkinje cell development.
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Overall design |
Laser capture microdissection was employed to obtain a highly enriched population of cerebellar Purkinje cells. Deep sequencing was performed on RNA isolated from 1000 Purkinje cells at five developmental timepoints (postnatal days P0, P4, P8, P14 and P21) in triplicate.
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Contributor(s) |
Clifford H, Dulneva A, Ponting CP, Haerty W, Becker EB |
Citation(s) |
30679692 |
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Submission date |
Sep 12, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Esther Becker |
E-mail(s) |
esther.becker@dpag.ox.ac.uk
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Organization name |
University of Oxford
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Street address |
Sherrington Road
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City |
Oxford |
ZIP/Postal code |
OX13PT |
Country |
United Kingdom |
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Platforms (1) |
GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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Samples (15)
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Relations |
BioProject |
PRJNA342640 |
SRA |
SRP089712 |