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Status |
Public on Apr 02, 2017 |
Title |
Decreased DEPTOR expression is involved in enhanced cell motility and invasion capability of ASS1 (Argininosuccinate synthetase 1) -KO cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To elucidate the mechanisms underlying the enhanced sensitivity to arginine in the absence of ASS1 expression in endometrial cancer cells, we performed DNA microarray-based gene expression profiling using HEC1B harboring the empty vector (EV) and ASS1-knockout (KO) HEC1B cells. Since accumulating evidence has shown that arginine regulates mTORC1 activity, we focused on the expression patterns of genes related to the mTOR complex. ASS1-KO HEC1B cells had lower DEPTOR transcript abundance than EV cells, while no significant difference was observed in the expression levels of genes coding for other mTOR complex components such as mTOR, RAPTOR, or RICTOR. Indeed, immunoblot analysis confirmed that the expression level of DEPTOR protein was significantly decreased in ASS1-KO HEC1B cells, which were cultured in arginine-replete conditions.
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Overall design |
The labeled cRNAs were hybridized on a Whole Human Genome Oligo Microarray ver. 2 (G4845A) in two dye swap experiments, resulting in four individual microarrays.
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Contributor(s) |
Ohshima K, Okuzaki D, Morii E |
Citation(s) |
28358054 |
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Submission date |
Sep 15, 2016 |
Last update date |
Jun 30, 2017 |
Contact name |
Daisuke Okuzaki |
E-mail(s) |
dokuzaki@biken.osaka-u.ac.jp
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Phone |
+81-6-6879-4935
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Organization name |
Osaka univ.
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Department |
Immunology Frontier Research Center
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Lab |
Human Immunology (Single Cell Genomics)
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Street address |
Yamadaoka 3-1
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City |
Suita |
State/province |
Osaka |
ZIP/Postal code |
565-0871 |
Country |
Japan |
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Platforms (1) |
GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
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Samples (4)
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GSM2311120 |
KO(cy3) vs EV(cy5) biological replicate 1 |
GSM2311121 |
EV(cy3) vs KO(cy5) biological replicate 1 dyeswap |
GSM2311122 |
KO(cy3) vs EV(cy5) biological replicate 2 |
GSM2311123 |
EV(cy3) vs KO(cy5) biological eplicate 2 dyeswap |
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Relations |
BioProject |
PRJNA343100 |