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| Status |
Public on Jul 12, 2017 |
| Title |
Analysis of splicing pattern and gene expression level of Matrin3 knockdown. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We identified RNA targets of Matrin3 using SH-SY5Y by PAR-CLIP analysis. Because Matrin3 mainly bound to intron of pre-mRNA, in order to find the effect of Matrin3 on splicing pattern and expression, we knocked down Matrin3 using SH-SY5Y cells by electroporation and extracted total RNAs from those cells. The total RNAs were subjected to whole transcripts microarray GeneChip® Affymetrix Human Transcriptome array 2.0.
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| Overall design |
SH-SY5Y cells were electroporated with Matrin3-siRNA or negative control siRNA. Forty eight hours after electroporation, total RNA was extracted and subjected to microarray analysis.
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| Contributor(s) |
Yamamoto M, Uemura Y, Kawahara Y |
| Citation(s) |
28695676 |
| Submission date |
Sep 15, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Yukio Kawahara |
| E-mail(s) |
ykawahara@rna.med.osaka-u.ac.jp
|
| Phone |
+81-6-6879-3827
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| Organization name |
Osaka University
|
| Department |
Graduate School of Medicine
|
| Lab |
Laboratory of RNA Function
|
| Street address |
2-2 Yamada-oka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
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| Platforms (2) |
| GPL17585 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version] |
| GPL17586 |
[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA343107 |
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