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| Status |
Public on Sep 01, 2017 |
| Title |
Crucial gene networks for fatty acid metabolism during lactation revealed by RNA-Sequencing of the goat mammary gland |
| Organism |
Capra hircus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Milk fatty acids secreted by the mammary glands are one of the most important determinants of the nutritional value of goat milk. However, there are few data available on the transcriptome-wide changes across lactation in the goat. In this study, goat mammary tissue from the periods encompassing lactation, cessation of milking and involution were used for digital gene expression sequencing (DGE) using the goat transcriptome as reference sequence. A total of 51299 unigenes were identified and annotated to 12763 genes, of which 9131 genes were differentially expressed (DEG) among the various stages of lactation. Functional classification through KOG, GO, and KEGG was used for selection of the top expressed genes and the differentially expressed genes (DEG). DEG series-cluster analysis revealed 16 possible expression patterns. Using co-expression analysis, within the lipid transport and metabolism KOG categories, PLA2, CPT1, PLD had the most number of correlated genes. GGA, SRPRB and AP4S1 were the top 3 with the highest number of correlated genes associated with intracellular trafficking, secretion, and vesicular transport. These data may provide candidate genes with a high probability of having functional roles in regulating of goat fatty acid metabolism during the various stages of lactation.
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| Overall design |
Examination of the crucial genes associated with fatty acid metabolism in mammary gland during lactation in dairy goat. SRP089750, PRJNA342566.
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| Contributor(s) |
Zhu J, Luo J |
| Citation missing |
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| BioProject |
PRJNA342566 |
| |
| Submission date |
Sep 19, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiangjiang Zhu |
| E-mail(s) |
zhujiang4656@hotmail.com
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| Organization name |
Southwest Uniersity for Nationalities
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| Department |
Institute of Qinghai-Tibetan Plateau
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| Street address |
#16, South Section, 1st Ring Road
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
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| Platforms (1) |
| GPL15473 |
Illumina HiSeq 2000 (Capra hircus) |
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| Samples (9)
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| GSM2322024 |
goat mammary gland from non-lactation period (K) RNA-Seq data rep2 |
| GSM2322025 |
goat mammary gland from non-lactation period (K) RNA-Seq data rep1 |
| GSM2322026 |
goat mammary gland from non-lactation period (K) RNA-Seq data |
| GSM2322027 |
goat mammary gland from dry-off (G) RNA-Seq data rep2 |
| GSM2322028 |
goat mammary gland from dry-off (G) RNA-Seq data rep1 |
| GSM2322029 |
goat mammary gland from dry-off (G) RNA-Seq data |
| GSM2322030 |
goat mammary gland from lactation period (R) RNA-Seq data rep 2 |
| GSM2322031 |
goat mammary gland from lactation period (R) RNA-Seq data rep 1 |
| GSM2322032 |
goat mammary gland from lactation period (R) RNA-Seq data |
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| Relations |
| SRA |
SRP089750 |
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