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Series GSE87180

Query DataSets for GSE87180

Status

Public on Jan 24, 2018

Title

ChIPseq in Pax7-reprogrammed corticotropes AtT-20 cells

Organism

Experiment type

Genome binding/occupancy profiling by high throughput sequencing

Summary

Deployment of a cell-specifying enhancer repertoire by the pioneer factor Pax7
The establishment and maintenance of cell identity depends on implementation of stable cell-specific chromatin landscapes. Pioneer transcription factors establish new cell fate competences by triggering chromatin remodeling during development. Here, we used pituitary cell specification to define the salient features of pioneer action. Comparison of purified pituitary cells of different lineages showed that chromatin accessibility differs at enhancers rather than promoters. The pioneer factor Pax7 specifies one pituitary lineage identity by opening a specific repertoire of enhancers that are distinct from the myogenic targets of Pax7. Pax7 binds its pioneer targets rapidly and days before chromatin remodeling and gene activation. Finally, enhancers opened by Pax7-dependent chromatin remodeling exhibit loss of DNA methylation and they acquire long term epigenetic memory. The present work identifies enhancer pioneering as a critical feature for cell fate specification and maintenance.

Overall design

ChIP assay followed by high throughput sequencing (ChIP-seq)

Contributor(s)

Mayran A, Gauthier Y, Drouin J

Citation(s)

29358650, 34197620

Submission date

Sep 21, 2016

Last update date

Jul 28, 2021

Contact name

ALEXANDRE MAYRAN

E-mail(s)

alexandre.mayran@epfl.ch

Organization name

EPFL

Street address

Station 19

City

LAUSANNE

State/province

Waadt

ZIP/Postal code

1015

Country

Switzerland

Platforms (1)

Illumina HiSeq 2000 (Mus musculus)

Samples (41)

More...

GSM2324086	Pax7 ChIPseq in AtT-20 expressing Pax7
GSM2324088	Tpit ChIPseq in AtT-20 expressing Pax7
GSM2324089	GR ChIPseq in AtT-20 expressing Neo (Dex+LIF)

GSM2324090	GR ChIPseq in AtT-20 expressing Pax7 (Dex+LIF)
GSM2324091	H3K4me1 ChIPseq in AtT-20 expressing Neo
GSM2324092	H3K4me1 ChIPseq in AtT-20 expressing Pax7
GSM2324093	p300 ChIPseq in AtT-20 expressing Neo
GSM2324094	p300 ChIPseq in AtT-20 expressing Pax7
GSM2324095	H3K27ac ChIPseq in AtT-20 expressing Neo
GSM2324096	H3K27ac ChIPseq in AtT-20 expressing Pax7
GSM2324097	H3 ChIPseq in AtT-20 expressing Neo
GSM2324098	H3 ChIPseq in AtT-20 expressing Pax7
GSM2324099	Stat3 ChIPseq in AtT-20 expressing Neo (Dex+LIF)
GSM2324100	Stat3 ChIPseq in AtT-20 expressing Pax7 (Dex+LIF)
GSM2324110	Pax7 ChIPseq in AtT-20 expressing ER-Pax7 (no ER-Pax7 induction)
GSM2324113	3x Flag ChIPseq in AtT-20 expressing Neo
GSM2324114	IgG ChIPseq in AtT-20
GSM2324116	Input of AtT-20
GSM2324118	Input of AtT-20 expressing Pax7
GSM2445269	Tpit ChIPseq in AtT-20
GSM2445270	Pax7 ChIPseq in AtT-20 expressing ERPax7 (30 minutes) (exo-like protocol)
GSM2445271	Pax7 ChIPseq in AtT-20 expressing ERPax7 (1h) (exo-like protocol)
GSM2445272	Pax7 ChIPseq in AtT-20 expressing ERPax7 (24h) (exo-like protocol)
GSM2445273	Pax7 ChIPseq in AtT-20 expressing ERPax7 (48h) (exo-like protocol)
GSM2445274	Pax7 ChIPseq in AtT-20 expressing ERPax7 (72h) (exo-like protocol)
GSM2445275	Pax7 ChIPseq in AtT-20 expressing Pax7 (exo-like protocol)
GSM2445276	Pax7 ChIPseq in AtT-20 expressing ERPax7 (stable induction followed by stable withdrawal)
GSM2445277	Stat3 ChIPseq in AtT-20 expressing ERPax7 (stable induction)
GSM2445278	Stat3 ChIPseq in AtT-20 expressing ERPax7 (stable induction followed by stable withdrawal)
GSM2684854	CTCF ChIPseq in AtT-20 expressing Neo
GSM2684855	SMC1 ChIPseq in AtT-20
GSM2684856	H3K9me3 ChIPseq in AtT-20 expressing Neo
GSM2684857	H3K9me3 ChIPseq in AtT-20 expressing Pax7
GSM2684858	H3K9me2 ChIPseq in AtT-20 expressing Neo
GSM2684859	H3K9me2 ChIPseq in AtT-20 expressing Pax7
GSM2684860	H3K27me3 ChIPseq in AtT-20 expressing Neo
GSM2684861	H3K27me3 ChIPseq in AtT-20 expressing Pax7
GSM2684862	H3K4me3 ChIPseq in AtT-20 expressing Neo
GSM2684863	H3K4me3 ChIPseq in AtT-20 expressing Pax7
GSM2806244	Ash2 ChIPseq in AtT-20 expressing Neo
GSM2806245	Ash2 ChIPseq in AtT-20 expressing Pax7

This SubSeries is part of SuperSeries:

Pioneer factor Pax7 deploys a stable enhancer repertoire for specification of cell fate

Relations

BioProject

SRA

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE87180_RAW.tar	30.7 Gb	(http)(custom)	TAR (of BIGWIG)
SRA Run Selector
Raw data are available in SRA
Processed data provided as supplementary file

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