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| Status |
Public on Nov 06, 2016 |
| Title |
Gene expression profiles of human hematopoietic progenitors [BLUEPRINT hematopoietic progenitors] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
An optimized whole genome bisulfite sequencing protocol (µWGBS, Farlik et al. 2015 Cell Reports) was used to establish DNA methylation profiles of FACS-purified stem and progenitor cells types of the human blood lineage. Most cell types were sorted from the peripheral blood of three donors each (43 donors total) with eight pools of ten cells, two pools of 50 cells, and one pool of 1000 cells. Additionally, two cell types (HSC, MPP) were sorted from bone marrow, fetal liver, and cord blood; single-cell methylome sequencing (scWGBS, Farlik et al. 2015 Cell Reports) was done for selected cell types (HSC, MPP, CMP, GMP, CLP, MLP0); and gene expression profiling using the Smart-seq2 protocol (Picelli et al. 2013 Nature Methods) was done on all stem/progenitor cell types (HSC, MPP, CMP, MEP, GMP, CLP, MLP0, MLP1, MLP2, MLP3).
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| Overall design |
Gene expression was analyzed for stem/progenitor cell types and terminally differentiated cell types of the human blood lineage (HSC, MPP, CMP, GMP, CLP, MLP0, MLP1, MLP2, MLP3).
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The raw sequencing data are available from EBI EGA under a data access agreement. Please contact the authors to access the raw data.
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| Web link |
http://blueprint-methylomes.computational-epigenetics.org/
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| Contributor(s) |
Farlik M, Halbritter F, Müller F, Frontini M, Bock C |
| Citation(s) |
27867036 |
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| Submission date |
Sep 21, 2016 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Christoph Bock |
| E-mail(s) |
cbock@cemm.oeaw.ac.at
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| Organization name |
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
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| Street address |
Lazarettgasse 14
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| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE87197 |
BLUEPRINT hematopoietic progenitors |
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| Relations |
| BioProject |
PRJNA343856 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE87195_rnaseq_ensT_all.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
| Raw data not provided for this record |
| Processed data are available on Series record |
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