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Series GSE87201

Query DataSets for GSE87201

Status

Public on Aug 29, 2017

Title

The transcriptome of human oocytes is related to age and ovarian reserve.

Organism

Experiment type

Expression profiling by array

Summary

Although it is well established that the ovarian reserve diminishes with increasing age, and that a woman’s age is correlated to lower oocyte quality, the interplay of a diminished reserve and age on oocyte developmental competence is not clear. After maturation, oocytes are mostly transcriptionally quiescent, and developmental competence prior to embryonic genome activation (EGA) relies on maternal RNA and proteins. Age and ovarian reserve both affects oocyte developmental competence, however, their relative importance in this process are difficult to tease out, as ageing is accompanied by a decrease in ovarian reserve. Oocytes store large quantities of RNA, including several noncoding transcripts (ncRNAs) involved in early development transcription and translation modulation. Despite the central role of ncRNAs in maternal to zygote transition, no characterization of the ncRNA transcriptome in human oocytes has been reported. This study aims at identifying how the human oocyte transcriptome changes across reproductive ages and ovarian reserve levels, with the goal of identifying candidate markers of developmental competence, and to assess the independent relevance of age and ovarian reserve in the changes of the transcriptome

Overall design

A total of 36 vitrified/warmed MII oocytes from 30 women undergoing oocyte donation were included in this study, processed and analyzed individually. Oocytes were divided in 4 groups, each including 9 oocytes, according to the woman´s age and antral follicular count (AFC) (mean ± SD): Young with High AFC (YH; age 21 ± 1 years and 24 ± 3 follicles)? Old with High AFC (OH; age 32 ± 2 years and 29 ± 7 follicles)? Young with Low AFC (YL; age 24 ± 2 years and 8 ± 2 follicles)? Old with Low AFC (OL; age 34 ± 1 years and 7 ± 1 follicles). Total RNA from each oocyte independently was isolated, amplified, labeled, and hybridized on HTA 2.0 arrays (Affymetrix). One sample from group Young with Low AFC (YL) was excluded due to apparent data quality issues. Remaining data were analyzed using TAC software and qPCR was performed to validate arrays.

Contributor(s)

Barragán M, Pons JI, Ferrer-Vaquer A, Schweitzer A, Hubbard J, Auer H, Rodolosse A, Vassena R

Citation(s)

Submission date

Sep 22, 2016

Last update date

Jun 15, 2020

Contact name

Montse BARRAGAN

E-mail(s)

mbarragan@eugin.es

Organization name

Clinica EUGIN

Street address

Baldiri Reixac, 15

City

Barcelona

State/province

Select One

ZIP/Postal code

08028

Country

Spain

Platforms (1)

[HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]

Samples (35)

More...

GSM2325104	Age 33-35 at donation + low ovarian reserve Sample 1 [D1.1]
GSM2325105	Age 33-35 at donation + low ovarian reserve Sample 1 [D2.1]
GSM2325106	Age 33-35 at donation + low ovarian reserve Sample 1 [D3.1]

GSM2325107	Age 33-35 at donation + low ovarian reserve Sample 1 [D4.1]
GSM2325108	Age 33-35 at donation + low ovarian reserve Sample 1 [D5.1]
GSM2325109	Age 33-35 at donation + low ovarian reserve Sample 1 [D6.1]
GSM2325110	Age 33-35 at donation + low ovarian reserve Sample 1 [D7.1]
GSM2325111	Age 33-35 at donation + low ovarian reserve Sample 1 [D8.1]
GSM2325112	Age 33-35 at donation + low ovarian reserve Sample 1 [D9.1]
GSM2325113	Age 20-22 at donation + low ovarian reserve Sample 1 [D11.1]
GSM2325114	Age 20-22 at donation + low ovarian reserve Sample 1 [D12.1]
GSM2325115	Age 20-22 at donation + low ovarian reserve Sample 1 [D13.1]
GSM2325116	Age 20-22 at donation + low ovarian reserve Sample 1 [D13.2]
GSM2325117	Age 20-22 at donation + low ovarian reserve Sample 1 [D14.1]
GSM2325118	Age 20-22 at donation + low ovarian reserve Sample 1 [D14.2]
GSM2325119	Age 20-22 at donation + low ovarian reserve Sample 1 [D15.1]
GSM2325120	Age 20-22 at donation + low ovarian reserve Sample 1 [D15.2]
GSM2325121	Age 20-22 at donation + high ovarian reserve Sample 1 [D16.1]
GSM2325122	Age 20-22 at donation + high ovarian reserve Sample 1 [D17.1]
GSM2325123	Age 20-22 at donation + high ovarian reserve Sample 1 [D17.2]
GSM2325124	Age 20-22 at donation + high ovarian reserve Sample 1 [D18.1]
GSM2325125	Age 20-22 at donation + high ovarian reserve Sample 1 [D19.1]
GSM2325126	Age 20-22 at donation + high ovarian reserve Sample 1 [D20.1]
GSM2325127	Age 20-22 at donation + high ovarian reserve Sample 1 [D21.1]
GSM2325128	Age 20-22 at donation + high ovarian reserve Sample 1 [D22.1]
GSM2325129	Age 20-22 at donation + high ovarian reserve Sample 1 [D23.1]
GSM2325130	Age 33-35 at donation + high ovarian reserve Sample 1 [D24.1]
GSM2325131	Age 33-35 at donation + high ovarian reserve Sample 1 [D25.1]
GSM2325132	Age 33-35 at donation + high ovarian reserve Sample 1 [D26.1]
GSM2325133	Age 33-35 at donation + high ovarian reserve Sample 1 [D26.2]
GSM2325134	Age 33-35 at donation + high ovarian reserve Sample 1 [D27.1]
GSM2325135	Age 33-35 at donation + high ovarian reserve Sample 1 [D28.1]
GSM2325136	Age 33-35 at donation + high ovarian reserve Sample 1 [D29.1]
GSM2325137	Age 33-35 at donation + high ovarian reserve Sample 1 [D30.1]
GSM2325138	Age 33-35 at donation + high ovarian reserve Sample 1 [D30.2]

Relations

BioProject

Download family	Format
SOFT formatted family file(s)	SOFT
MINiML formatted family file(s)	MINiML
Series Matrix File(s)	TXT

Supplementary file	Size	Download	File type/resource
GSE87201_RAW.tar	861.7 Mb	(http)(custom)	TAR (of CEL)
Processed data included within Sample table

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