|
| Status |
Public on Sep 24, 2016 |
| Title |
Expression data from hematopoietic stem cells from Zeb2-KO (Zeb2Δ/ΔMx1-Cre) mice versus WT mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Zeb2 has been shown to influence hematopoietic differentiation and loss thereof leads to complex differentiation defects in multiple hematopoietic lineages.
We used microarrays to compare the expresssion profile of Zeb2-defective with WT hematopoietic stem cells and identified several dinstict classes of up-regulated genes during this process.
|
| |
|
| Overall design |
A population of cells highly enriched for hematopoietic stem cells (LKS-Slam marker positive cells) was sorted by flow cytometry from bone marrow of (Zeb2Δ/ΔMx1-Cre) mice and WT mice eight weeks after induction with pIC. Total RNA was RNA extracted and hybridised on Affymetrix microarrays. We used three distict biological replicates from each the Zeb2Δ/ΔMx1-Cre cells versus WT (control) cells.
|
| |
|
| Contributor(s) |
Janzen V |
| Citation(s) |
27683414 |
| |
| Submission date |
Sep 23, 2016 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Viktor Janzen |
| E-mail(s) |
janzen.viktor@gmail.com
|
| Organization name |
University of Bonn
|
| Street address |
Sigmund-Freud-Str. 25
|
| City |
Bonn |
| ZIP/Postal code |
53127 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA344304 |
Less...
More...