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Status |
Public on Sep 24, 2016 |
Title |
Expression data from hematopoietic stem cells from Zeb2-KO (Zeb2Δ/ΔMx1-Cre) mice versus WT mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Zeb2 has been shown to influence hematopoietic differentiation and loss thereof leads to complex differentiation defects in multiple hematopoietic lineages. We used microarrays to compare the expresssion profile of Zeb2-defective with WT hematopoietic stem cells and identified several dinstict classes of up-regulated genes during this process.
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Overall design |
A population of cells highly enriched for hematopoietic stem cells (LKS-Slam marker positive cells) was sorted by flow cytometry from bone marrow of (Zeb2Δ/ΔMx1-Cre) mice and WT mice eight weeks after induction with pIC. Total RNA was RNA extracted and hybridised on Affymetrix microarrays. We used three distict biological replicates from each the Zeb2Δ/ΔMx1-Cre cells versus WT (control) cells.
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Contributor(s) |
Janzen V |
Citation(s) |
27683414 |
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Submission date |
Sep 23, 2016 |
Last update date |
Mar 04, 2019 |
Contact name |
Viktor Janzen |
E-mail(s) |
janzen.viktor@gmail.com
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Organization name |
University of Bonn
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Street address |
Sigmund-Freud-Str. 25
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City |
Bonn |
ZIP/Postal code |
53127 |
Country |
Germany |
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Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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Samples (6)
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Relations |
BioProject |
PRJNA344304 |