|Public on Dec 31, 2016
|DUX4-induced dsRNA and MYC mRNA Stabilization Activate Apoptotic Pathways in Human Cell Models of Facioscapulohumeral Dystrophy
|Expression profiling by high throughput sequencing
|Facioscapulohumeral muscular dystophy (FSHD) is caused by the mis-expression of DUX4 in skeletal muscle cells. DUX4 is a transcription factor that activates genes normally associated with stem cell biology and its mis-expression in FSHD cells results in apoptosis. To identify genes and pathways necessary for DUX4-mediated apoptosis, we performed an siRNA screen in an RD rhabdomyosarcoma cell line with an inducible DUX4 transgene. Our screen identified components of the MYC-mediated apoptotic pathway and the double-stranded RNA (dsRNA) innate immune response pathway as mediators of DUX4-induced apoptosis. Further investigation revealed that DUX4 expression led to increased MYC mRNA, accumulation of nuclear dsRNA foci, and activation of the dsRNA response pathway in both RD cells and human myoblasts. Nuclear dsRNA foci were associated with aggregation of the exon junction complex component EIF4A3. The elevation of MYC mRNA, dsRNA accumulation, and EIF4A3 nuclear aggregates in FSHD muscle cells suggest that these processes might contribute to FSHD pathophysiology.
|RNA-seq of human rhabdomyosarcoma cells with an inducible DUX4 transgene (RD-DUX4i) cells was performed on RNA samples collected from 3 separate experimental replicates induced from 16-18 hours with doxycycline, as indicated and compared to the respective uninduced condition.
|Shadle SC, Zhong JW, Campbell AE, Conerly ML, Jagannathan S, Wong C, Morello TD, Tapscott SJ
|Sep 29, 2016
|Last update date
|May 15, 2019
|Fred Hutch Cancer Research Center
|1100 Fairview N. Ave
|Illumina HiSeq 2500 (Homo sapiens)