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Status |
Public on Jun 12, 2018 |
Title |
Sequencing-based analyses characterize a tumor suppressive role of mir-1271 repressed by DNA hypermethylation in gastric cancer |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing Methylation profiling by high throughput sequencing
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Summary |
To identify tumor suppressive microRNAs repressed by DNA hypermethylation in gastric cancer (GC), we analyzed methylome and miRNome of EpCAM+/CD44+ GC cells. Among a set of microRNAs hypermethylated and downregulated in GC, mir-1271 was uncovered as a microRNA repressed by DNA hypermethylation in GC. Forced expression of mir-1271 significantly suppressed growth, migration, and invasion of GC cells both in vitro and in vivo. To identify target genes and cancer signaling pathways regulated by mir-1271, we examined differentially-expressed genes responsive to mir-1271 by performing RNA-sequencing.
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Overall design |
2 paired normal and GC cancer cells miRNA-seq, 3 GC cell lines MBD-seq, miR-1271 mimic treated GC cells and control cells RNA-seq
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Contributor(s) |
Lim B, Baek S, Kim J, Kim S, Kim YS, Kim M |
Citation(s) |
29862663 |
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Submission date |
Oct 10, 2016 |
Last update date |
Jul 25, 2021 |
Contact name |
Jong Hwan Kim |
E-mail(s) |
kkjjhhk@kribb.re.kr
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Phone |
0821093299557
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Organization name |
Korea Research Institute of Bioscience and Biotechnology
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Department |
Genome structure research center
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Street address |
125 Gwahak-ro, Yuseong-gu
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City |
Daejeon |
ZIP/Postal code |
ASI|KR|KS015|DAEJEON |
Country |
South Korea |
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Platforms (2) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (19)
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Relations |
BioProject |
PRJNA347596 |
SRA |
SRP091347 |