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| Status |
Public on Sep 04, 2007 |
| Title |
Comparative expression analysis between sigE (TTHB211) deletion mutant and wild-type of T. thermophilus HB8 |
| Organism |
Thermus thermophilus HB8 |
| Experiment type |
Expression profiling by array
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| Summary |
We observed the expression profile of the total mRNA of sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type.
Keywords: cell type comparison
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| Overall design |
Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 8 h, and then crude RNA was extracted. In order to compare mRNA expression in the sigE (TTHB211) deletion mutant with that of wild type, the expression of each mRNA at the time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.
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| Contributor(s) |
Shinkai A, Kashihara A, Kuramitsu S, Yokoyama S |
| Citation(s) |
17905996 |
| |
| Submission date |
Aug 14, 2007 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Akeo Shinkai |
| E-mail(s) |
ashinkai@spring8.or.jp, y_agari@spring8.or.jp
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| URL |
http://www.srg.harima.riken.jp/
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| Organization name |
RIKEN Harima Institute
|
| Department |
SPring-8 Center
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| Street address |
1-1-1, Kouto, Sayo
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| City |
Hyogo |
| ZIP/Postal code |
679-5148 |
| Country |
Japan |
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| Platforms (1) |
| GPL4902 |
Riken Thermus thermophilus HB8 4K mRNA array |
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| Samples (6)
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| GSM218190 |
TTHB211 deletion mutant strain_8h_1 |
| GSM218191 |
TTHB211 deletion mutant strain_8h_2 |
| GSM218192 |
TTHB211 deletion mutant strain_8h_3 |
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| This SubSeries is part of SuperSeries: |
| GSE8781 |
SuperSeries for the study of expression analysis of the T. thermophilus sigE (TTHB211) |
| GSE10548 |
SuperSeries for the study of expression analysis of the T. thermophilus sigE (TTHB211) and anti-sigE (TTHB212) |
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| Relations |
| BioProject |
PRJNA105293 |
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