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| Status |
Public on Oct 01, 2008 |
| Title |
Mechanisms underlying hypoxia tolerance in Drosophila melanogaster: hairy as a metabolic switch |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
Hypoxia-induced cell injury has been related to multiple pathological conditions. In order to render hypoxia-sensitive cells and tissues resistant to low O2 environment, in this current study, we used Drosophila melanogaster as a model to dissect the mechanisms underlying hypoxia-tolerance. A D. melanogaster strain that lives perpetually in an extremely low-oxygen environment (4% O2, an oxygen level that is equivalent to that over about 4,000 m above Mt. Everest) was generated through laboratory selection pressure using a continuing reduction of O2 over many generations. This phenotype is genetically stable since selected flies, after several generations in room air, survive at this low O2 level. Gene expression profiling showed striking differences between tolerant and naïve flies, in larvae and adults, both quantitatively and qualitatively. Up-regulated genes in the tolerant flies included signal transduction pathways (e.g., Notch and Toll/Imd pathways), but metabolic genes were remarkably down-regulated in the larvae. Furthermore, a different allelic frequency and enzymatic activity of the triose phosphate isomerase (TPI) was present in the tolerant versus naïve flies. The transcriptional suppressor, hairy, was up-regulated in the microarrays and its binding elements were present in the regulatory region of the specifically down-regulated metabolic genes but not others, and mutations in hairy significantly reduced hypoxia tolerance. We conclude that, the hypoxia-selected flies: (a) altered their gene expression and genetic code, and (b) coordinated their metabolic suppression, especially during development, with hairy acting as a metabolic switch, thus playing a crucial role in hypoxia-tolerance.
Keywords: genetic bases of hypoxia adaptation
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| Overall design |
27 isogenic D. melanogaster Lines were pooled and following long-term selection over generations with decreased oxygen level in the culture environment. The differences in gene expression were compared between adapted flies and generation matched naive controls by microarray. Pooled RNA samples from 3rd instar larvae of 27 parental lines were used as common reference.
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| Contributor(s) |
Zhou D, Xue J, Lai JC, Schork NJ, White KP, Haddad GG |
| Citation(s) |
18927626 |
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| Submission date |
Aug 16, 2007 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Dan Zhou |
| E-mail(s) |
d2zhou@ucsd.edu
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| Phone |
858-822-6889
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| Fax |
858-534-6971
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| Organization name |
University of California, San Diego, School of Medicine
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| Department |
Pediatrics
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| Lab |
Haddad Lab
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| Street address |
CMG 103, 9500 Gilman Drive, MC0735
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0735 |
| Country |
USA |
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| Platforms (2) |
| GPL3833 |
YUSM (K. White) D. melanogaster cDNA array |
| GPL5733 |
YUSM (K. White) D. melanogaster cDNA array (II) |
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| Samples (29)
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| GSM112183 |
D. Melanogaster_control_18th generation_12-1 |
| GSM112185 |
D.mel_Control_18th Generation_12-3 |
| GSM112187 |
D.mel_Control_18th Generation_12-5 |
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| Relations |
| BioProject |
PRJNA102111 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8803_RAW.tar |
24.3 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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