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| Status |
Public on Aug 01, 2008 |
| Title |
Overexpression of the Apoptotic Cell Removal Receptor, MERTK, in Alveolar Macrophages of Cigarette Smokers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Mononuclear phagocytes play an important role in the removal of apoptotic cells by expressing cell surface receptors that recognize and remove apoptotic cells. Based on the knowledge that cigarette smoking is associated with increased lung cell turnover, we hypothesized that alveolar macrophages (AM) of normal cigarette smokers may exhibit enhanced expression of apoptotic cell removal receptor genes. AM obtained by bronchoalveolar lavage of normal non-smokers (n=11) and phenotypic normal smokers (n=13, 36 ± 6 pack-yr) were screened for mRNA expression of all known apoptotic cell removal receptors using Affymetrix HG-U133 Plus 2.0 chips with TaqMan RT-PCR confirmation. Of the 14 known apoptotic receptors expressed, only MER Tyrosine Kinase (MERTK), a transmembrane tyrosine kinase receptor, was significantly up-regulated in smokers. MERTK expression was then assessed in AM of smokers vs nonsmokers by TaqMan RT-PCR, immunohistochemistry, Western and flow analysis. Smoker AM had up-regulation of MERTK mRNA levels (smoker vs non-smoker, 3.6-fold by microarray, p<0.003; 9.5-fold by TaqMan RT-PCR, p<0.02). Immunohistochemistry demonstrated a qualitative increase in MERTK protein expression on AM of smokers. Increased protein expression of MERTK on AM of smokers was confirmed by Western and flow analyses (p< 0.007 and p< 0.0002, respectively). MERTK, a cell surface receptor that recognizes apoptotic cells, is expressed on human AM, and its expression is up-regulated in AM of cigarette smokers. This may reflect an increased demand for removal of apoptotic cells in smokers, an observation with implications for the development of chronic obstructive pulmonary disease (COPD), a disorder associated with dysregulated apoptosis of lung parenchymal cells.
Keywords: Comparison of gene expression in alveolar macrophages of normal non-smokers and normal smokers.
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| Overall design |
Alveolar macrophages were obtained from bronchoalveolar lavage.
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| Contributor(s) |
Kazeros A, Harvey B, Carolan BJ, Vanni H, Krause A, Crystal RG |
| Citation(s) |
18587056 |
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| Submission date |
Aug 20, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Yael Strulovici-Barel |
| E-mail(s) |
yas2003@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
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| Department |
Department of Genetic Medicine
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| Lab |
Crystal
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| Street address |
1300 York Avenue
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (24)
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| GSM219241 |
alveolar macrophages, non-smoker 001 |
| GSM219242 |
alveolar macrophages, non-smoker 002 |
| GSM219243 |
alveolar macrophages, non-smoker 003 |
| GSM219244 |
alveolar macrophages, non-smoker 005 |
| GSM219245 |
alveolar macrophages, non-smoker 006 |
| GSM219246 |
alveolar macrophages, non-smoker 008 |
| GSM219247 |
alveolar macrophages, non-smoker 013 |
| GSM219248 |
alveolar macrophages, non-smoker 014 |
| GSM219249 |
alveolar macrophages, non-smoker 019 |
| GSM219250 |
alveolar macrophages, non-smoker 021 |
| GSM219251 |
alveolar macrophages, non-smoker 035 |
| GSM219252 |
alveolar macrophages, smoker 001 |
| GSM219253 |
alveolar macrophages, smoker 002 |
| GSM219254 |
alveolar macrophages, smoker 023 |
| GSM219255 |
alveolar macrophages, smoker 025 |
| GSM219256 |
alveolar macrophages, smoker 027 |
| GSM219257 |
alveolar macrophages, smoker 033 |
| GSM219258 |
alveolar macrophages, smoker 040 |
| GSM219259 |
alveolar macrophages, smoker 041 |
| GSM219260 |
alveolar macrophages, smoker 044 |
| GSM219261 |
alveolar macrophages, smoker 057 |
| GSM219262 |
alveolar macrophages, smoker 058 |
| GSM219263 |
alveolar macrophages, smoker 063 |
| GSM219264 |
alveolar macrophages, smoker 069 |
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| Relations |
| BioProject |
PRJNA102147 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE8823_RAW.tar |
517.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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