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| Status |
Public on May 19, 2017 |
| Title |
Transcriptional signature of SPCH mutants compromised in the bHLH domain in response to brassinosteroids |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
In order to describe the molecular phenotype of spch-5 and to explore possible changes in gene expression underlying the effects on its epidermal development of BL treatment, we performed a microarray-based transcriptomic analysis. Using 3-days old seedlings grown in the absence or presence of BL, we compared the transcriptomic profiles of wild type (Col-0) and spch-5 plants. In addition, we analyzed a SPCHPPP-complemented spch-3 line to identify gene expression changes distinct of spch-5 or common to the two SPCH variants. We thus obtained six data sets (3 genotypes in two growth conditions).
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| Overall design |
3 days-old Arabidopsis plants grown in mock medium (DMSO) and brassinosteroids treatment (BL 50 nM) were used. Three genotypes were used: Col-0, spch-5 and SPCHPPP
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| Contributor(s) |
de Marcos A, Mena M, Fenoll C |
| Citation(s) |
28507175 |
| |
| Submission date |
Oct 19, 2016 |
| Last update date |
May 21, 2017 |
| Contact name |
Alberto de Marcos Serrano |
| E-mail(s) |
albertode.marcos@uclm.es
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| Organization name |
Universidad de Castilla-La Mancha
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| Street address |
Av carlos III s/n, Edificio Sabatini Lab 0.8 F.V
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| City |
Toledo |
| State/province |
Toledo |
| ZIP/Postal code |
45071 |
| Country |
Spain |
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| Platforms (1) |
| GPL22511 |
Agilent-045560 Arabidopsis thaliana 8x60k v1_complete |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA349173 |
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