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| Status |
Public on Oct 22, 2016 |
| Title |
Quant Seq analysis of primary stretched rat alveolar type I-like epithelial (AEC-I) cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Rat AEC-I cell monolayers were generated from primary isolated type-II pneumoncytes by plating them on fibronectin-coated silastic membranes. After 4 days of culturing, type-II pneumocytes trasform into AEC-I cells and form monolayers.
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| Overall design |
Monolayers were randomized in the following four conditions:
1) unstretched monolayers (NS).
2) Monolayers exposed to 25% surface area change with custom built biaxial cyclic (15/min) stretcher for 6 hours (S).
3) Unstretched monolayers pretreated with 1microM GSK2606414 PERK kinase inhibitor for 18 hours (NS_PI).
4) Stretched monolayers pretreated with 1micro M GSK2606414 for 18 hours (S_PI).
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| |
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| Contributor(s) |
Dolinay T, Shumyatcher M, Himes BE |
| Citation(s) |
28363030 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| T32 HL007586 |
Training in Pulmonary Immunology |
UNIVERSITY OF PENNSYLVANIA |
Steven Mark Albelda |
|
| Submission date |
Oct 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Tamas Dolinay |
| E-mail(s) |
tdolinay@hotmail.com
|
| Phone |
4125278764
|
| Organization name |
University of Pennsylvania
|
| Department |
Bioengineering
|
| Lab |
Susan Margulies
|
| Street address |
220 S. 33rd St., Towne building, Room 390
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL20084 |
Illumina NextSeq 500 (Rattus norvegicus) |
|
| Samples (20)
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| Relations |
| BioProject |
PRJNA349816 |
| SRA |
SRP091911 |
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