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Status |
Public on Dec 05, 2016 |
Title |
Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs [siRNA-Seq] |
Organism |
Schizosaccharomyces pombe |
Experiment type |
Non-coding RNA profiling by high throughput sequencing
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Summary |
Cellular quiescence (G0) is a ubiquitous stress response through which cells enter a state of reversible dormancy, acquiring distinct properties including reduced metabolism, resistance to stress and long life. G0 entry involves dramatic changes to the chromatin landscape and transcriptional program of cells, but the mechanisms coordinating these processes remain poorly understood. Using the fission yeast, here we track the G0-associated chromatin and transcriptional changes temporally. We show that as cells enter G0, their survival and global gene expression programs become increasingly dependent on Clr4/SUV39H, the sole histone H3 lysine 9 (H3K9) methyltransferase, and RNA interference (RNAi) proteins. Notably, G0 entry results in RNAi-dependent H3K9 methylation of several euchromatic regions, prior to which Argonaute 1-associated small RNAs from these regions emerge. Overall our data reveal a new function for constitutive heterochromatin proteins in establishing the global G0 transcriptional program and suggest that stress-induced alterations in Argonaute-associated sRNAs can specify the deployment of transcriptional regulatory proteins to unique sites in eukaryotic genomes.
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Overall design |
Comparison of Ago1-associated small RNA in wt at 0 and 24h into N starvation (3 replicates)
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Contributor(s) |
Joh RI, Khanduja JS, Calvo IA, Mistry M, Palmieri CM, Savol AJ, Sui SJ, Sadreyev RI, Aryee MJ, Motamedi M |
Citation(s) |
27984744 |
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Submission date |
Oct 25, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Richard Joh |
E-mail(s) |
rich.i.joh@gmail.com
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Organization name |
Mass General Hospital
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Department |
Center for Cancer Research
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Street address |
149 13th St
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City |
Charlestown |
State/province |
MA |
ZIP/Postal code |
02129 |
Country |
USA |
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Platforms (1) |
GPL17225 |
Illumina HiSeq 2500 (Schizosaccharomyces pombe) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE89151 |
Survival in quiescence requires the euchromatic deployment of Clr4/SUV39H by Argonaute-associated small RNAs |
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Relations |
BioProject |
PRJNA350506 |
SRA |
SRP092042 |