|
| Status |
Public on Aug 01, 2008 |
| Title |
Gene expression changes with inducible expression of activated ras and ras effector domain mutants |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The goals of this study were to identify gene transcription changes in cells expressing activated H-RasV12 or activated versions of the ras effector domain mutants RasV12G37, RasV12S35, and RasV12C40 for the purpose of identifying common or unique transcription alterations in cells transformed by different ras signal transduction pathways.
Keywords: genetic modification design
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| |
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| Overall design |
HME16C mammary epithelial cells infected with pLRT control vector, or H-RasV12-, V12G37-, V12S35-, or V12C40-expressing vectors were each treated with 250ng/mL doxycycline and total RNA prepared at two separate times to give two biological replicates. Then dye swap experiments were performed with each biological replicate.
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| |
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| Citation(s) |
18597688 |
| |
| Submission date |
Aug 30, 2007 |
| Last update date |
Aug 29, 2019 |
| Contact name |
Kathleen Kelly |
| E-mail(s) |
kk62j@nih.gov
|
| Phone |
3014354652
|
| Fax |
3014354655
|
| Organization name |
National Institutes of Health
|
| Department |
Cancer and Cell Biology Branch
|
| Lab |
Kelly
|
| Street address |
37 Convent Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
|
| Samples (16)
|
| GSM225968 |
G37 vs. pLRT - repeat 1 - mAdbID:48887 |
| GSM225969 |
G37 vs. pLRT - repeat 2 - mAdbID:48888 |
| GSM225970 |
G37 vs. pLRT - repeat 3 - mAdbID:49118 |
| GSM225971 |
G37 vs. pLRT - repeat 4 - mAdbID:49119 |
| GSM225972 |
V12 vs. pLRT - repeat 1 - mAdbID:50811 |
| GSM225973 |
V12 vs. pLRT - repeat 2 - mAdbID:50812 |
| GSM225974 |
V12 vs. pLRT - repeat 3 - mAdbID:50813 |
| GSM225975 |
V12 vs. pLRT - repeat 4 - mAdbID:50814 |
| GSM225976 |
S35 vs. pLRT - repeat 1 - mAdbID:51255 |
| GSM225977 |
S35 vs. pLRT - repeat 2 - mAdbID:51256 |
| GSM225978 |
S35 vs. pLRT - repeat 3 - mAdbID:51257 |
| GSM225979 |
S35 vs. pLRT - repeat 4 - mAdbID:51258 |
| GSM225980 |
C40 vs. pLRT - repeat 1 - mAdbID:51259 |
| GSM225981 |
C40 vs. pLRT - repeat 2 - mAdbID:51260 |
| GSM225982 |
C40 vs. pLRT - repeat 3 - mAdbID:51261 |
| GSM225983 |
C40 vs. pLRT - repeat 4 - mAdbID:51262 |
|
| Relations |
| BioProject |
PRJNA102317 |
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