|
| Status |
Public on Dec 13, 2016 |
| Title |
Transcriptional responses of Rhizopus microsporus host and non-host isolates to physical interaction with Burkholderia endobacteria of the host |
| Organism |
Rhizopus microsporus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this study was to identify fungal gene expression changes during early stages of symbiosis establishment with its Burkholderia endosymbionts. Results: Leveraging this RNA-seq dataset we identified fungal genes utilized for symbiosis establishment with bacteria.
|
| |
|
| Overall design |
Methods: Host (ATCC 52813) and non-host (ATCC 11559) isolates of R. microsporus were growth alone or in the presence of Burkholderia endobacteria isolated from the host isolate. Fungal mycelia were harvested at a timepoint when the fungal colony has just begun to come into physical contact with the bacteria. Total RNA was extracted, rRNA depleted with RiboZero, and sequenced on the Illumina Hi-Seq 100bp-paired end reads platform
|
| |
|
| Contributor(s) |
Lastovetsky OA, Pawlowska TE |
| Citation(s) |
27956601 |
| |
| Submission date |
Oct 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Olga Lastovetsky |
| E-mail(s) |
ol57@cornell.edu
|
| Organization name |
Cornell University
|
| Street address |
334 Plant Science Building, 236 Tower Rd, Cornell University
|
| City |
Ithaca |
| State/province |
NY |
| ZIP/Postal code |
14850 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL22615 |
Illumina HiSeq 2500 (Rhizopus microsporus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA351268 |
| SRA |
SRP092261 |
Less...
More...