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Status |
Public on Dec 13, 2016 |
Title |
Transcriptional responses of Rhizopus microsporus host and non-host isolates to physical interaction with Burkholderia endobacteria of the host |
Organism |
Rhizopus microsporus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The goal of this study was to identify fungal gene expression changes during early stages of symbiosis establishment with its Burkholderia endosymbionts. Results: Leveraging this RNA-seq dataset we identified fungal genes utilized for symbiosis establishment with bacteria.
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Overall design |
Methods: Host (ATCC 52813) and non-host (ATCC 11559) isolates of R. microsporus were growth alone or in the presence of Burkholderia endobacteria isolated from the host isolate. Fungal mycelia were harvested at a timepoint when the fungal colony has just begun to come into physical contact with the bacteria. Total RNA was extracted, rRNA depleted with RiboZero, and sequenced on the Illumina Hi-Seq 100bp-paired end reads platform
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Contributor(s) |
Lastovetsky OA, Pawlowska TE |
Citation(s) |
27956601 |
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Submission date |
Oct 28, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Olga Lastovetsky |
E-mail(s) |
ol57@cornell.edu
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Organization name |
Cornell University
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Street address |
334 Plant Science Building, 236 Tower Rd, Cornell University
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City |
Ithaca |
State/province |
NY |
ZIP/Postal code |
14850 |
Country |
USA |
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Platforms (1) |
GPL22615 |
Illumina HiSeq 2500 (Rhizopus microsporus) |
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Samples (12)
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Relations |
BioProject |
PRJNA351268 |
SRA |
SRP092261 |