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Series GSE89511 Query DataSets for GSE89511
Status Public on Jan 01, 2017
Title CD86 regulates a pro-survival signal in myeloma cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary While multiple myeloma patient prognosis has improved over the past decade, research towards discovery of new therapeutic avenues is important, and could lead to a cure for this chronic plasma cell malignancy. Data analysis from a myeloma patient database shows that the CD28-CD86 signaling module may provide a survival advantage in myeloma cells that negatively impacts patient outcome. Here we show that blocking the CD28-CD86 pathway, by silencing or with CTLA-4-Ig, leads to myeloma cell death. Blockade of this pathway leads to downregulation of nutrient transporters, integrins, and IRF4, a known myeloma survival factor. Our data also indicate that CD86, the canonical "ligand" in this pathway, is mediating a pro-survival signal via the cytosolic domain that has not been previously described. These findings indicate that blockade of this pathway is a promising therapeutic avenue for myeloma, as it leads to modulation of different processes important in cell viability.
Overall design The myeloma cell lines MM.1s, RPMI8226, KMS18, and RPMI8226 that overexpresses the anti-apoptotic protein Mcl1 were transfected with short hairpin RNAs targeting CD28, CD86, Gapdh (control), or empty vector (pLK0.1) control and gene expression analysis was performed by RNA-seq.
Contributor(s) Gavile CM, Barwick BG, Lawrence BH
Citation(s) 29296880
Submission date Nov 03, 2016
Last update date Mar 28, 2022
Contact name Benjamin G Barwick
Phone (404) 285-2964
Organization name Emory University
Department Hematology and Medical Oncology
Lab Barwick Lab
Street address 1365 Clifton Rd. NE WCI-C 4th Floor Benches 36-37
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (16)
GSM2375013 MM1S_Vec
GSM2375014 MM1S_Gapdh
GSM2375015 MM1S_CD28
BioProject PRJNA352410
SRA SRP092559

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89511_diff.geneFpkm.exon.glm.LogFc.0.exc.Gapdh.RPMI8226_MCL1.txt.gz 2.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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