|
| Status |
Public on Dec 31, 2017 |
| Title |
Convergent exaptation of Alu and B/ID SINEs for Staufen-mediated mRNA decay |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Identifying orthologous mRNAs subject to SMD in both mouse and human myoblasts using siRNA depletion of Staufen or Upf1
|
| |
|
| Overall design |
mRNAs subject to Staufen-mediated decay should increase in level upon depletion of either Staufen or Upf1. Cultured myoblasts (mouse or human) treated with control, Staufen, or Upf1 siRNAs were extracted and RNA was purifed, polyA selected and sequenced. Changes in level were detected by DESEQ analysis.
|
| |
|
| Contributor(s) |
Bronwyn L, Manuel A, Lily S, Sol K, Lynne M |
| Citation(s) |
29339519 |
| Submission date |
Nov 07, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Manuel Ares |
| E-mail(s) |
ARES@UCSC.EDU
|
| Phone |
831 459-4628
|
| Organization name |
UC Santa Cruz
|
| Department |
MCDB
|
| Lab |
Ares
|
| Street address |
1156 High Street
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA352669 |
| SRA |
SRP092751 |
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