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| Status |
Public on Apr 01, 2017 |
| Title |
Array comparative genomic hybridization of spheres derived from TH-MYCN (hemizygote) mice [Agilent-027441] |
| Organism |
Mus musculus |
| Experiment type |
Genome variation profiling by array
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| Summary |
It has been already reported that there are undifferentiated/proliferating neuroblasts in the postnatal sympathetic ganglia in TH-MYCN mice, a neuroblastoma model. We established suitable spheroid culture condition that selectively isolates undifferentiated neuroblasts from superior mesenteric ganligon (SMG) of TH-MYCN mice. In order to investigate the chromosomal alterations (gains or losses) of spheres derived from TH-MYCN mice, we carried out array comparative genomic hybridization. We investigated whether chromosomal alterations occured during early neuroblastoma tumorigenesis in TH-MYCN mice.
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| Overall design |
Primary spheres were cultured from SMG of E13.5 and 3-week-old TH-MYCN mice, and from tumor tissues of TH-MYCN mice. Eight biological replicates of primary spheres from tumor tissues of TH-MCYN mice were subjected to the array CGH. Passageable spheres were obtained by subculturing primary spheres derived from E13.5 and 3-week-old TH-MYCN SMG. Six biological replicates of E13.5 TH-MYCN spheres and eight biological replicates of 3-week-old TH-MYCN spheres were subjected to array CGH.
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| Contributor(s) |
Tsubota S, Ohira M, Kadomatsu K |
| Citation(s) |
28807939 |
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| Submission date |
Nov 10, 2016 |
| Last update date |
Sep 13, 2017 |
| Contact name |
Shoma Tsubota |
| E-mail(s) |
tsubota@med.nagoya-u.ac.jp
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| Organization name |
Nagoya University Graduate School of Medicine
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| Department |
Department of Molecular Biology
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| Street address |
65 Tsurumai-cho, Showa-ku
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| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
466-8550 |
| Country |
Japan |
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| Platforms (1) |
| GPL17131 |
Agilent-027411 SurePrint G3 Mouse CGH Microarray 4x180K (Probe Name version) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE89741 |
Array comparative genomic hybridization of tissue developed in TH-MYCN (hemizygote) mice |
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| Relations |
| BioProject |
PRJNA353068 |
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