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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2023 |
| Title |
A novel reciprocal paracrine signaling loop mediated by transient expression of EphrinB2 in the myocardium regulates ventricular wall formation. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Genome-wide gene expression studies on EphB4 mutant over wild-type mice. We used microarray to show enrichment of neuregulin-1 pathway in EphB4 mutant mice
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| Overall design |
A total of 6 mice were used, including 3 EphB4 mutant and 3 wild-type. Total RNA was isolated using Qiagen RNeasy kit (Qiagen, Valencia, CA) from mouse heart. Ten micrograms of total RNA was used to synthesize cDNA. T7 promoter introduced during the first strand synthesis was then used to direct cRNA synthesis, which was labeled with biotinylated deoxynucleotide triphosphate, following the manufacturer's protocol (Affymetrix, San Diego, CA). After fragmentation, the biotinylated cRNA was hybridized to Mouse 430.2 Array chips (Affymetrix, San Diego, CA)
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| Contributor(s) |
Liu J, Ma B, Zaggar SJ, Punj V, Gill PS, Sucov HM, Kumar SR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 16, 2016 |
| Last update date |
Mar 01, 2023 |
| Contact name |
Vasu Punj |
| E-mail(s) |
vasupunj@gmail.com
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| Organization name |
USC
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| Street address |
Biggy Street
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA354272 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE89905_RAW.tar |
21.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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