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| Status |
Public on Apr 18, 2018 |
| Title |
Single-cell sequencing of stage 4 chicken embryos |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Abstract from Vermillion et al: During vertebrate development, progenitor cells give rise to tissues and organs through a complex choreography that commences at gastrulation. A hallmark event of gastrulation is the formation of the primitive streak, a linear assembly of cells along the anterior-posterior (AP) axis of the developing organism. To examine the primitive streak at a single-cell resolution, we measured the transcriptomes of individual chick cells from the streak or the surrounding tissue (the rest of the area pellucida) in Hamburger-Hamilton stage 4 embryos. The single-cell transcriptomes were then ordered by the statistical method Wave-Crest to deduce both the relative position along the AP axis and the prospective lineage of single cells. The ordered transcriptomes reveal intricate patterns of gene expression along the primitive streak.
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| |
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| Overall design |
Examination of single-cells of stage 4 chicken embryos.
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| |
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| Contributor(s) |
Vermillion K, Vereide D |
| Citation(s) |
29678445 |
| |
| Submission date |
Nov 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
David Vereide |
| E-mail(s) |
dvereide@morgridge.org
|
| Organization name |
Morgridge Institute for Research
|
| Department |
Regenerative Biology
|
| Street address |
330 North Orchard Street
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
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| Samples (205)
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| Relations |
| BioProject |
PRJNA354269 |
| SRA |
SRP131342 |
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