|
| Status |
Public on Jan 01, 2018 |
| Title |
Protointrons in Saccharomyces sp. |
| Organisms |
Saccharomyces bayanus; Saccharomyces cerevisiae; Saccharomyces mikatae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We sequenced non-ribosomal RNA from a upf1 deletion mutant of S. cerevisiae, and from wild type S. mikatae and S. bayanus, and identified a new class of yeast introns.
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| |
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| Overall design |
Yeast non-ribosomal RNAs were isolated from vegetatively growing cells or cells treated with rapamycin for 60 minutes to mimick starvation, RNA was sequenced, and splice junctions were identified and validated.
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| |
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| Contributor(s) |
Talkish J, Igel H, Perriman RJ, Shiue L, Katzman S, Munding EM, Shelansky R, Donohue J, Ares M Jr |
| Citation(s) |
31437148, 32459805, 38585890 |
| |
| Submission date |
Nov 21, 2016 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Jason Talkish |
| E-mail(s) |
jtalkish@ucsc.edu
|
| Phone |
831-459-4917
|
| Organization name |
University of California, Santa Cruz
|
| Department |
Molecular, Cellular, and Developmental Biology
|
| Lab |
Ares Lab, Sinsheimer Rm. 412
|
| Street address |
1156 High St.
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platforms (3) |
| GPL21656 |
Illumina HiSeq 4000 (Saccharomyces cerevisiae) |
| GPL22698 |
Illumina HiSeq 4000 (Saccharomyces mikatae) |
| GPL22699 |
Illumina HiSeq 4000 (Saccharomyces bayanus) |
|
| Samples (8)
|
| GSM2398349 |
cerevisiae upf1D untreated replicate A |
| GSM2398350 |
cerevisiae upf1D untreated replicate B |
| GSM2398351 |
cerevisiae upf1D rapa-treated replicate A |
|
| Relations |
| BioProject |
PRJNA354419 |
| SRA |
SRP093610 |
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