|
| Status |
Public on Jun 26, 2017 |
| Title |
Inhibition of RNA polymerase II allows controlled mobilisation of retrotransposons for plant breeding [rice] |
| Organism |
Oryza sativa Japonica Group |
| Experiment type |
Other
|
| Summary |
We report the application of a high-throughput sequencing approach that is specifically designed to analyze the active mobilome of eukaryotes after simultaneous inhibition of RNA polymerase II and/or DNA methylation in O. sativa japonica. We found that the simultaneous inhibition of Pol II and DNA-methylation activated the evolutionary young Houba-Retrotransposon in O. sativa suggesting a crucial role of Pol II in regulating retrotransposition in plants.
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| Overall design |
Comparison of the mobilomes in 10-day-old rice seedlings after inhibition of Pol II and/or DNA-methylation compared to control conditions.
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| Contributor(s) |
Thieme M, Lanciano S, Mirouze M, Bucher E, Daccord N, Balzergue S |
| Citation(s) |
28687080 |
| |
| Submission date |
Nov 23, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Etienne Bucher |
| E-mail(s) |
etienne.bucher@angers.inra.fr
|
| Phone |
++33 2 41 22 57 97
|
| Organization name |
IRHS
|
| Lab |
Bucher
|
| Street address |
42 rue Georges Morel
|
| City |
Beaucouzé |
| ZIP/Postal code |
49071 |
| Country |
France |
| |
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| Platforms (1) |
| GPL22703 |
Illumina MiSeq (Oryza sativa Japonica Group) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA354815 |
| SRA |
SRP093854 |
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