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| Status |
Public on Aug 06, 2020 |
| Title |
Transcriptome analysis of the induced hepatocyte-like (iHep) cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have found three specific combinations of two transcription factors, comprising Hnf4alpha plus Foxa1, Foxa2 or Foxa3, could convert mouse embryonic fibroblasts (MEFs) into cells that closely resemble hepatocytes in vitro. Then we used RNA-seq to explore the conversion event to the iHep cells.
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| Overall design |
RNA-Seq for 6 iHep_4a1 cells (Foxa1/Hnf4alpha), 4 iHep_4a2 cells (Foxa2/Hnf4alpha), 6 iHep_4a3 cells (Foxa3/Hnf4alpha), 6 cells 48 hours after transfection (48hr_4a1, 48hr_4a2, 48hr_4a3), 2 iMEFs transduced with Foxa3 del2 mutant/Hnf4alpha, 2 MEFs, and 2 hepatocytes (Hep).
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| Contributor(s) |
Suzuki A, Ohkawa Y, Nagasaki M, Horisawa K, Ueno K |
| Citation(s) |
32755593 |
| |
| Submission date |
Nov 25, 2016 |
| Last update date |
Aug 10, 2020 |
| Contact name |
Atsushi Suzuki |
| Organization name |
Kyushu University
|
| Department |
Medical Institute of Bioregulation
|
| Lab |
Division of Organogenesis and Regeneration
|
| Street address |
3-1-1 Maidashi, Higashi-ku
|
| City |
Fukuoka |
| State/province |
Fukuoka |
| ZIP/Postal code |
812-8582 |
| Country |
Japan |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA355155 |
| SRA |
SRP094003 |
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