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| Status |
Public on Jul 13, 2017 |
| Title |
Identifying ASCL1-mediated chromatin changes in primary GBM stem cell cultures [ATAC-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ASCL1 mediates neuronal differentiation of GBM stem cell (GSC) cultures. We sought to identify chromatin changes upon induced ASCL1 expression in primary human GSC cultures. In this dataset, we include ATAC-seq data obtained from GSC cultures harbouring a CRISPR-deletion of ASCL1. We assessed differential ASCL1 binding between control and GSC cultures induced to overexpress ASCL1 after 14 days of doxycycline treatment.
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| Overall design |
Glioblastoma cells with a genetic knockout of ASCL1 is compared to genetic knockout of ASCL1 overexpressing ASCL1 (n = 2 biological replicates; total = 4 samples)
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| |
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| Contributor(s) |
Park NI, Desai K, Dirks PB |
| Citation(s) |
28712938 |
| |
| Submission date |
Nov 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter B Dirks |
| Organization name |
The Hospital for Sick Children
|
| Department |
Arthur and Sonia Labatt Brain Tumour Research Centre and Division of Neurosurgery
|
| Street address |
686 Bay St
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 0A4 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA354999 |
| SRA |
SRP093971 |
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