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| Status |
Public on Mar 13, 2008 |
| Title |
Fine mapping of regulatory loci for mammalian gene expression using radiation hybrids II |
| Platform organism |
Mus musculus |
| Sample organisms |
Homo sapiens; Cricetulus griseus |
| Experiment type |
Expression profiling by array
|
| Summary |
We have mapped regulatory loci for nearly all protein coding genes in the mammalian genome using microarray measurements from a panel of mouse/hamster radiation hybrids.
Proof of principle transfection experiment. Pcdh7 (a gene identified as being a regulator of many genes) is transfected into A23 and HEK cells and compared to A23 and HEK cells transfected with an empty vector. Upregulated genes predicted by our approach are compared with the transfection experiments
Keywords: tranfection, regulation of gene expression
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| Overall design |
There are 4 microarrays. The first two are biological replicates of A23 (transfected with Pcdh7, labeled with cy5) / A23 (tranfected with empty vector, labeled with cy3). The second two are biological replicates of HEK (transfected with Pcdh7, labeled with cy5) / HEK (tranfected with empty vector, labeled with cy3).
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| Contributor(s) |
Park CC, Smith DJ |
| Citation(s) |
18362883, 19521529 |
| |
| Submission date |
Sep 17, 2007 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Christopher Park |
| Phone |
310-948-7917
|
| Organization name |
UCLA
|
| Department |
Molecular and Medical Pharmacology
|
| Lab |
Desmond Smith
|
| Street address |
23-151 CHS, 650 Charles E. Young Drive
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2872 |
Agilent-012694 Whole Mouse Genome G4122A (Feature Number version) |
|
| Samples (4)
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| GSM230013 |
A23 (transfected with Pcdh7, cy5) / A23 (tranfected with empty vector, cy3), biological replica 1 |
| GSM230014 |
A23 (transfected with Pcdh7, cy5) / A23 (tranfected with empty vector, cy3), biological replica 2 |
| GSM230015 |
HEK (transfected with Pcdh7, cy5) / HEK (tranfected with empty vector, cy3), biological replica 1 |
| GSM230016 |
HEK (transfected with Pcdh7, cy5) / HEK (tranfected with empty vector, cy3), biological replica 2 |
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| This SubSeries is part of SuperSeries: |
| GSE9052 |
Fine mapping of regulatory loci for mammalian gene expression using radiation hybrids |
|
| Relations |
| BioProject |
PRJNA105325 |
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