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| Status |
Public on Jun 01, 2018 |
| Title |
Reconstitution of the human pancreatic niche stimulates differentiation of hESCs into beta cells and reveals new signals for pancreatic endocrine cell maturation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA-seq was performed for hESC-derived endocrine progenitors (EPs) treated with WNT5A for short-term (12h) and long-term (5 days) to investigate the downstream targets of WNT5A in EPs. Sequencing of untreated cells and those treated with WNT5A for 12h, which are at EP stage, and for 5 days, which are at beta cell stage, allowed for observation of developmental changes during in vitro differentiation.
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| |
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| Overall design |
Two controls as 12h or 5 days untreated (maintained in B27) and two 12h or 5 days WNT5A-treated EPs.
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| |
|
| Contributor(s) |
Yang D, Chmieloweic J, Scavuzzo MA |
| Citation(s) |
35414140 |
| |
| Submission date |
Dec 01, 2016 |
| Last update date |
May 05, 2022 |
| Contact name |
Malgorzata Borowiak |
| E-mail(s) |
borowiak@bcm.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
1 Baylor Plaza
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA355891 |
| SRA |
SRP094450 |
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