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| Status |
Public on Oct 25, 2007 |
| Title |
PIWI-associated piRNA in Drosophila melanogaster ovary |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Heterochromatin, representing the silenced state of transcription, largely consists of transposon-enriched and highly repetitive sequences. Implicated in heterochromatin formation and transcriptional silencing in Drosophila are PIWI and repeat-associated small interfering RNAs (rasiRNAs). Despite this, the role of PIWI in rasiRNA expression and heterochromatic silencing remains unknown. Here we report the identification and characterization of 12,903 PIWI-interacting RNAs (piRNAs) in Drosophila, demonstrating that rasiRNAs represent a subset of piRNAs.
Keywords: PIWI, piRNA, epigenetic regulation, heterochromatin
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| Overall design |
PIWI-associated small RNA cDNA library was sequenced for one time by high-throughput 454 pyrosequencing. Putative small RNA sequences were extracted and BLAST against the Drosophila melanogaster genome release 5. Presented here is a list of non-redundant PIWI-associated small RNAs, which have at least one genome match determined by BLASTn.
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| Contributor(s) |
Yin H, Lin H |
| Citation(s) |
17952056, 33609367 |
| |
| Submission date |
Sep 23, 2007 |
| Last update date |
Apr 13, 2021 |
| Contact name |
Hang Yin |
| E-mail(s) |
hang.yin@duke.edu
|
| Phone |
(203)7856215
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| Organization name |
Yale University Stem Cell Center
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| Department |
Department of Cell Biology
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| Lab |
Haifan Lin laborotary
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| Street address |
10 Amistead Street, Room 220
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| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06509 |
| Country |
USA |
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| Platforms (1) |
| GPL5922 |
PIWI-associated piRNA in Drosophila melanogaster ovary |
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| Samples (1) |
| GSM231091 |
PIWI-associated piRNA in Drosophila melanogaster ovary (454 Pyrosequencing, 2006) |
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| Relations |
| BioProject |
PRJNA102681 |
