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| Status |
Public on Jun 12, 2017 |
| Title |
DNA methylation profiling in lungs tissues of smokers and patients with COPD |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by array
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| Summary |
Epigenetics changes have been shown to be affected by cigarette smoking. It is possible that cigarette smoke (CS)-mediated DNA methylation would affect several cellular and pathophysiological processes, acute exacerbations, and comorbidity in lungs of patients with chronic obstructive pulmonary disease (COPD). We sought to determine whether genome-wide lung DNA methylation profiles of smokers and patients with COPD were significantly different from non-smokers. We isolated DNA from lung tissues of patients including 8 lifelong non-smokers, 8 current smokers, and 8 patients with COPD, and subsequently analyzed the samples using the Illumina’s Infinium HumanMethylation450 BeadChip.
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| Overall design |
We performed Genome-Wide DNA methylation analysis using lung parenchyma tissue DNA samples from Non-smokers (NS), Smokers (S) and patients with COPD (C) in 2 separate batches. In the first batch of samples, we used Sample ID# 1-4 in each of the three groups: Non-smokers (NS.1, NS.2, NS.3, NS.4), Smokers (S.1, S.2, S.3, S.4) and COPD (C.1, C.2, C.3, C.4). The second batch of samples, we used additional samples in each of the three groups (Sample ID# 5-8): Non-smokers (NS.5, NS.6, NS.7, NS.8), Smokers (S.5, S.6, S.7, S.8) and patients with COPD (C.5, C.6, C.7, C.8).
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| Contributor(s) |
Sundar IK, Rahman I |
| Citation(s) |
28416970 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HL085613 |
Mechanisms of cigarette smoke-induced chromatin remodeling in lung inflammation: MECHANISMS OF CIGARETTE SMOKE-INDUCED LUNG CELLULAR SENESCENCE: MECHANISMS OF CIGARETTE SMOKE-INDUCED LUNG CELLULAR SENESCENCE: MECHANISMS OF CIGARETTE SMOKE-INDUCED LUNG CELLULAR SENESCENCE: MECHANISMS OF CIGARETTE SMOKE-INDUCED LUNG CELLULAR SENESCENCE: MECHANISMS OF CIGARETTE SMOKE-INDUCED LUNG CELLULAR SENESCENCE: MECHANISMS OF CIGARETTE SMOKE-INDUCED LUNG CELLULAR SENESCENCE |
UNIVERSITY OF ROCHESTER |
RAHMAN |
| P30 ES001247 |
TRACE CONTAMINANTS AS ENVIRONMENTAL HEALTH HAZARDS: Environmental Agents as Modulators of Disease Processes: TRACE CONTAMINANTS AS ENVIRONMENTAL HEALTH HAZARDS: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: Environmental Agents as Modulators of Disease Processes: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: Environmental Agents as Modulators of Disease Processes: ENVIRONMENTAL AGENTS AS MODULATORS OF DISEASE PROCESSES: Environmental Agents as Modulators of Disease Processes |
UNIVERSITY OF ROCHESTER |
Deborah A Cory-Slechta |
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| Submission date |
Dec 16, 2016 |
| Last update date |
Mar 22, 2019 |
| Contact name |
Isaac Kirubakaran Sundar |
| E-mail(s) |
isaackirubakaran@gmail.com
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| Phone |
585-748-6042
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| Organization name |
University of Rochester
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| Department |
Environmental Medicine
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| Street address |
601 Elmwood Ave, Box 850
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| City |
Rochester |
| State/province |
New York |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL13534 |
Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA357831 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE92511_RAW.tar |
183.1 Mb |
(http)(custom) |
TAR |
| GSE92511_unnormalized_Methylation_data.txt.gz |
58.3 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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