|
| Status |
Public on Dec 23, 2016 |
| Title |
Mechanisms involved in porcine early embryo survival following ethanol exposure |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
|
| Summary |
We aimed to determine the toxicogenomic impacts and the mechanisms involved in preimplantation embryonic survival following 0.2% ethanol exposure in porcine embryos. Gene expression changes were measured with a porcine embryo specific microarray and confirmed by RT-qPCR. Compared to control, ethanol exposure led to a 43% decrease in blastocyst rate and activated pathways associated with oxidative stress and nervous system damage, such as TP53 and TGF.
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| |
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| Overall design |
4 pools of 10 control porcine blastocysts were compared to 4 pools of 10 ethanol-exposed porcine blastocysts in a microarray experiment.
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| |
|
| Contributor(s) |
Page-Lariviere F, Campagna C, Sirard M |
| Citation(s) |
28069986 |
| |
| Submission date |
Dec 20, 2016 |
| Last update date |
Mar 28, 2017 |
| Contact name |
Florence Pagé-Larivière |
| E-mail(s) |
florence.page-lariviere.1@ulaval.ca
|
| Organization name |
Laval University
|
| Street address |
2440, boulevard Hochelaga
|
| City |
Québec |
| State/province |
Québec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL17779 |
Agilent-031068 EmbryoGene Porcine Array Version 1 (EMPV1) [Probe version] |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA358585 |
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