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Series GSE92936 Query DataSets for GSE92936
Status Public on Jan 31, 2019
Title Roles of Structural maintenance of chromosome flexible domain containing 1 (Smchd1) in early lineage formation and development in mice
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The function of Structural maintenance of chromosome flexible domain containing 1 (Smchd1) was examined during mouse preimplantation development using an siRNA knockdown approach. Transient SMCHD1 deficiency during the period between fertilization and morula/early blastocyst stage compromised embryo viability and resulted in reduced cell number, reduced embryo diameter, and reduced nuclear volumes at the morula stage. RNAseq analysis of Smchd1 knockdown morulae revealed aberrant increases in expression of mRNAs related to the trophoblast lineage, indicating SMCHD1 inhibits trophoblast lineage gene expression and promotes inner cell mass formation. siRNA knockdown also reduced expression of cell proliferation genes, including S-phase kinase-associated protein 2 (Skp2). Smchd1 expression was elevated in Caudal type homeobox transcription factor 2 (Cdx2)-/- blastocysts, indicating enriched expression, and further indicating a role in inner cell mass development. These results indicate that Smchd1 plays dual roles in the preimplantation embryo, promoting a lineage-appropriate pattern of gene expression supporting inner cell mass formation, whilst controlling lineage formation and gene expression in the trophectoderm.
 
Overall design Effects of SMCHD1 siRNA knockdown were tested in mouse embryos
 
Contributor(s) Midic U, Vincent KA, Wang K, Lokken A, Severance AL, Ralston A, Knott JG, Latham KE
Citation(s) 29900695
Submission date Dec 27, 2016
Last update date May 15, 2019
Contact name Uros Midic
E-mail(s) uros@msu.edu
Organization name Michigan State University
Department Animal Sciences
Lab Latham lab
Street address 474 S. Shaw Lane
City East Lansing
State/province MI
ZIP/Postal code 48824-1225
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM2440706 Control_siRNA1
GSM2440707 Control_siRNA2
GSM2440708 Control_siRNA3
Relations
BioProject PRJNA359109
SRA SRP095702

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE92936_FPKMs.fpkm.txt.gz 1.2 Mb (ftp)(http) TXT
GSE92936_gene_exp.diff.gz 1.3 Mb (ftp)(http) DIFF
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Raw data are available in SRA
Processed data are available on Series record

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