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| Status |
Public on Dec 27, 2017 |
| Title |
Effect of RBP2 on MCF7 breast cancer cells (RNA-seq) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
RNA-sequencing analysis of RBP2 overexpressing MCF7 cell lines. RBP2 (also known as JARID1A), a member of the JARID1 family of histone H3 lysine K4 demethylases, has been considered to have an oncogenic potential in several cancer including breast cancer. Results provide insight into the transcriptional regulation of RBP2 in estrogen receptor positve breast cancer.
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| Overall design |
MCF7 cells stably infected with lentiviruses encoding either control (pLVX vector) or RBP2 (pLVX-RBP2) were cultured in phenol-red free DMEM containt 10% FBS for 48h. Total RNA was isolated from the cultures using Trizol reagent. For each of the 2 conditions, 3 biological replicates were included. In total, 6 RNA-sequencin samples were analyzed; 3 controls and 3 RBP2 overexpressing cells.
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| Contributor(s) |
Choi H, Kong G |
| Citation(s) |
29028222 |
| BioProject |
PRJNA356680 |
| |
| Submission date |
Dec 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hee-Joo Choi |
| E-mail(s) |
wing180@naver.com
|
| Organization name |
Hanyang University
|
| Street address |
222 Yangshimri-ro, Seongdonggu
|
| City |
Seoul |
| ZIP/Postal code |
04763 |
| Country |
South Korea |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| SRA |
SRP094834 |
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