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Series GSE93033 Query DataSets for GSE93033
Status Public on Jan 01, 2017
Title Genome-wide maps of H3K27 state in transfected NIH3T3 cells.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Epigenetic H3k27me3 changes of mouse NIH3T3 cells comparing control NIH3T3 cells transfected with a pEFm6-BRAF with cells transfected with pEFm6-BRAFV600E. By obtaining over 30 Million bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of BRAF transfected. We find that global lysine 3 and lysine 27 trimethylation levels changed under the condition of activated ERK by BRAFV600E, and specific genes might be downregulated for the hight level of H3K27me3 induced by BRAFV600E
Overall design Examination global levels of H3K27me3 in the 2 transfected cells.
Contributor(s) Hou P, Qu Y
Citation(s) 28656062
Submission date Dec 30, 2016
Last update date Oct 24, 2019
Contact name Yiping Qu
Organization name The First Affiliated Hospital of Xi’an Jiaotong University
Department Department of Endocrinology
Street address The First Affiliated Hospital of Xi’an Jiaotong University
City Xi’an
ZIP/Postal code 701181
Country China
Platforms (1)
GPL9185 Illumina Genome Analyzer (Mus musculus)
Samples (2)
GSM2442819 H3K27me3_ChIPSeq - wild type
GSM2442820 H3K27me3_ChIPSeq - mutant
BioProject PRJNA359491
SRA SRP095854

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Supplementary file Size Download File type/resource
GSE93033_RAW.tar 5.2 Mb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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