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| Status |
Public on Jan 01, 2017 |
| Title |
Genome-wide maps of H3K27 state in transfected NIH3T3 cells. |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Epigenetic H3k27me3 changes of mouse NIH3T3 cells comparing control NIH3T3 cells transfected with a pEFm6-BRAF with cells transfected with pEFm6-BRAFV600E. By obtaining over 30 Million bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of BRAF transfected. We find that global lysine 3 and lysine 27 trimethylation levels changed under the condition of activated ERK by BRAFV600E, and specific genes might be downregulated for the hight level of H3K27me3 induced by BRAFV600E
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| Overall design |
Examination global levels of H3K27me3 in the 2 transfected cells.
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| Contributor(s) |
Hou P, Qu Y |
| Citation(s) |
28656062 |
| |
| Submission date |
Dec 30, 2016 |
| Last update date |
Oct 24, 2019 |
| Contact name |
Yiping Qu |
| E-mail(s) |
15398036489@163.com
|
| Organization name |
The First Affiliated Hospital of Xi’an Jiaotong University
|
| Department |
Department of Endocrinology
|
| Street address |
The First Affiliated Hospital of Xi’an Jiaotong University
|
| City |
Xi’an |
| ZIP/Postal code |
701181 |
| Country |
China |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA359491 |
| SRA |
SRP095854 |
