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Series GSE9306 Query DataSets for GSE9306
Status Public on Oct 31, 2007
Title Short RNA profiling of ES cells with and without Dicer
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by array
Summary To better understand Dicer function, and the potential mechanisms by which RNAi contributes to pluripotency, short RNA expression was profiled from 4 ES cell libraries: 2 made from a Dicer-conditional ES cell line before and several months after deletion of Dicer, and 2 made before and 5 days after demethylation of J1 ES cells with 5-aza-deoxycytidine. Contributed here are the unannotated sequence data from each library, including those sequences that did not match the mouse genome or any known non-coding RNAs.
Keywords: 454 sequencing, miRNA, dicer, demethylation
Overall design cDNA libraries were made as described in Neilson et al. Genes and Development, 2007 Mar 1;21(5):578-89.
Contributor(s) Calabrese JM, Seila AC, Yeo GW, Sharp PA
Citation(s) 17989215
Submission date Oct 12, 2007
Last update date Mar 17, 2012
Contact name J. Mauro Calabrese
Phone 617 253 6457
Organization name MIT
Department Biology
Lab Sharp
Street address 40 Ames Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL5986 MIT/SharpLab Mouse ES cell 454 2007
Samples (4)
GSM237107 J1 ES cells untreated
GSM237108 J1 ES cells 5-aza-deoxycytidine
GSM237109 Dicer-deleted ES cell line
BioProject PRJNA102957

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Processed data included within Sample table

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