|
| Status |
Public on Oct 31, 2007 |
| Title |
Short RNA profiling of ES cells with and without Dicer |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by array
|
| Summary |
To better understand Dicer function, and the potential mechanisms by which RNAi contributes to pluripotency, short RNA expression was profiled from 4 ES cell libraries: 2 made from a Dicer-conditional ES cell line before and several months after deletion of Dicer, and 2 made before and 5 days after demethylation of J1 ES cells with 5-aza-deoxycytidine. Contributed here are the unannotated sequence data from each library, including those sequences that did not match the mouse genome or any known non-coding RNAs.
Keywords: 454 sequencing, miRNA, dicer, demethylation
|
| |
|
| Overall design |
cDNA libraries were made as described in Neilson et al. Genes and Development, 2007 Mar 1;21(5):578-89.
|
| |
|
| Contributor(s) |
Calabrese JM, Seila AC, Yeo GW, Sharp PA |
| Citation(s) |
17989215 |
| |
| Submission date |
Oct 12, 2007 |
| Last update date |
Mar 17, 2012 |
| Contact name |
J. Mauro Calabrese |
| E-mail(s) |
mauroc@mit.edu
|
| Phone |
617 253 6457
|
| Organization name |
MIT
|
| Department |
Biology
|
| Lab |
Sharp
|
| Street address |
40 Ames Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5986 |
MIT/SharpLab Mouse ES cell 454 2007 |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA102957 |
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