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| Status |
Public on Feb 27, 2018 |
| Title |
Identification of miRNAs that regulate vestibular compensation |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
To investigate which miRNAs regulate the vestibular compensation after unilateral vestibular deafferentiation (UVD), we have performed microarray for miRNAs as a discovery platform. UVD induces breakdown of the activity of ipsilesional vestibular nuclei and an unbalance of activity between bilateral vestibular nuclei. Vestibular compensation is a course of rebalancing of activities of bilateral vestibular nuclei. It takes place mainly in medial vestibular nucleus. This study was performed using seven week-old-male Sprague–Dawley rats. Based on our previous experiment about vestibular compensation course, we set two time points for harvesting medial vestibular nuclei: 4hr and 4 days after unilateral vestibular deafferentiation. Twenty four animals were divided into two experimental groups: UVD group undergoing UVD at left side (n = 12); and SO group undergoing sham operation (SO) at left side (n=12). Six animals of each group were anesthetized deeply and euthanized at 4 hr or 4 days after surgery, respectively. Medial vestibular nucleus at left side was harvested. Medial vestibular nucleus from three animals became one sample for microarray. Sequentially two samples were obtained for each time point in one group. Microarray for miRNAs was performed using the Agilent Rat miRNA Microarray 8x15K platforms. Considering the fold change of normalized signal intensities between two time points in UVD group and between UVD and SO groups at the same time, miR-31a-5p, 133a-3p, 133b-3p, 204-5p, 206-3p, 218a-5p, 219a-5p, 221-3p and 497-5p were selected as the candidate miRNAs. This result was validated by quantitative reverse transcription-PCR.
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| Overall design |
Unilateral vestibular deafferentiation induced miRNAs expression in medial vestibular nucleus of Sprague–Dawley rat was measured at 4hr and 4 days after unilateral vestibular deafferentiation. Comparing these results with the results from the sham operation group, the candidate miRNAs that might regulate vestibular compensation were selected.
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| Contributor(s) |
Chang MY, Park MK |
| Citation(s) |
28821887 |
| |
| Submission date |
Jan 04, 2017 |
| Last update date |
Feb 27, 2018 |
| Contact name |
Munyoung Chang |
| E-mail(s) |
caduent@gmail.com
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| Organization name |
Chung-Ang University College of Medicine
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| Department |
Otorhinolaryngology-Head and Neck Surgery
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| Street address |
102 Heukseok-ro, Dongjak-gu
|
| City |
Seoul |
| ZIP/Postal code |
06973 |
| Country |
South Korea |
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| Platforms (1) |
| GPL22760 |
Agilent-070154 Rat miRNA Microarray (miRNA ID version version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA360079 |
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