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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 05, 2017 |
| Title |
Genomic instability during reprogramming by nuclear transfer is DNA replication dependent |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
SNP genotyping by SNP array
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| Summary |
Somatic cells can be reprogrammed to a pluripotent state by nuclear transfer into oocytes, yet developmental arrest often occurs. While incomplete transcriptional reprogramming is known to cause developmental failure, reprogramming also involves concurrent changes in gene expression, cell cycle progression and nuclear structure. Here we study cellular reprogramming events in human and mouse nuclear transfer (NT) embryos prior to embryonic genome activation. We show that genetic instability marked by frequent chromosome segregation errors and DNA damage arise prior to, and independent of, transcriptional activity. These errors occur following transition through DNA replication and are repaired by BRCA1. In the absence of mitotic nuclear remodeling, DNA replication is delayed and errors are exacerbated in subsequent mitosis. These results demonstrate that independent of gene expression, cell type-specific features of cell cycle progression constitute a barrier sufficient to prevent the transition from one cell type to another during reprogramming.
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| Overall design |
16 Samples
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| Contributor(s) |
Chia G, Agudo J, Treff NR, Sauer MV, Billing D, Brown B, Baer R, Egli D |
| Citation(s) |
28263958 |
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| Submission date |
Jan 04, 2017 |
| Last update date |
Jul 30, 2019 |
| Contact name |
Nathan R Treff |
| E-mail(s) |
NTreff@rmanj.com
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| Phone |
973-871-1236
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| Organization name |
RMA of NJ
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| Department |
Molecular Biology
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| Lab |
Suite 100
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| Street address |
111 Madison Ave
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| City |
Morristown |
| State/province |
NJ |
| ZIP/Postal code |
07960 |
| Country |
USA |
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| Platforms (1) |
| GPL3718 |
[Mapping250K_Nsp] Affymetrix Mapping 250K Nsp SNP Array |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA360093 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE93123_RAW.tar |
426.9 Mb |
(http)(custom) |
TAR (of CEL, CNT) |
| Processed data provided as supplementary file |
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